Sru Biosystems, Inc.

Nexcelom Bioscience LLC

X-body, Inc.

Timothy Smith

Peter Li

Lance Gavin Laing

Bo Lin

Jean Qiu

Gangadhar Jogikalmath

Christine C Genick

Lara Madison

Leo L Chan

Todd Sobolewski

William C Karl

Kevin Flanagan

John Gerstenmeier, Iii

Alnoor Pirani

Justin R Hascup

Nicholas N Shaw

John Gerstenmaier, Iii

Kurt Albertson

Sarah Kessel

Oliver Dery

Li Peter

The invention provides novel sample chambers, sample analysis units, multi-well plates, and imaging and analytical systems as well as methods for accurate, efficient and high-throughput imaging, measurement and analysis of diverse types of biological cells to obtain information such as cell count, cell size, cell concentration, cell sub-population, cell morphology, cell viability, etc.

System and method for cell imaging, analysis and measurement

The invention generally relates to analytical and monitoring systems useful for analyzing and measuring cells and biological samples. More particularly, the invention provides systems and methods for internal calibration and focus reference for cytometry imaging.

Internal focus reference beads for imaging cytometry

The present invention generally relates to systems and methods for counting biomolecules or cells. In certain embodiments, the invention provides a cell counting or biomolecule counting system including: a covered chamber having a known height and configured to hold a suspension of biomolecules or cells in a sample; at least one fluorescent light source connected to at least one fluorescent light beam narrowing device; a bright-field light source connected to a bright-field light beam narrowing device; a microscope objective; a detection device; a fluorescent filter assembly to allow only excitation light to illuminate the sample and allow only emission light from the sample to be imaged by the detection device; and a movable light shutter to block bright-field light during fluorescent detection.

Systems and methods for counting cells and biomolecules

The invention provides methods of detecting a change in cell growth patterns, methods of screening many different antibodies in one receptacle, and methods of detecting specific binding of an antibody to a protein or cell, wherein the antibody is in a mixture of many different antibodies.

Methods for screening cells and antibodies

A device for simultaneously dispensing a solution containing a sample to a test device and aspirating the sample from the device. In one embodiment the device is hand-held and includes a control mechanism, e.g., button for activating the dispensing and aspiration of the sample. The testing device may take the form of a multi-well plate with a plurality of wells arranged in rows and columns, with the bottom of the wells formed as a photonic crystal biosensor. The device can be configured with dispense and aspirate manifolds and associated dispense and aspirate ports which are positioned in all the wells along a row or column of wells so as to simultaneously aspirate and dispense solution to all the wells in a row or column of the multi-well plate.

Simultaneous aspirator and dispenser for multiwell plates and similar devices

A method of making a measurement of binding affinity of a sample comprises the steps of: introducing the sample into a well having a bottom formed as a photonic crystal biosensor, wherein a portion of the sample becomes bound to the biosensor; making a measurement of the change in the shift in peak wavelength value as a function of time (k) from the well as the sample is introduced into the well; simultaneously dispensing the sample to the well and aspirating the sample from the well and measuring the change in the shift in peak wavelength value as a function of time (k) during the simultaneous dispensing and aspirating, and calculating an equilibrium association constant or an equilibrium dissociation constant for the sample from the values of kand k.

The invention provides compositions and methods of detecting binding of molecules to a biosensor. Compositions of the invention comprise a multiwell plate with an integrated biosensor and a membrane.

Multiwell plates with integrated biosensors and membranes

Growing community of inventors

Dracut, MA, United States of America

Timothy Smith