Applied Biosystems, LLC

The Perkin-elmer Corporation

Applera Corporation

Fluidigm Corporation

Integenx, Inc.

Perkin-elmer Corp., Applied Biosystems Division

Timothy M Woudenberg

Robert P Ragusa

Reid Burton Kowallis

Emily S Winn-Deen

Yefim M Raysberg

Michael S Albin

Muhammad A Sharaf

John Girdner Atwood

Khairuzzaman Bashar Mullah

Enrico G Picozza

Richard A Leath

Albert Carmelo Mossa

Lisa May Goven

Fenton Williams

Clive Miles

David H Tracy

Will Bloch

Lawrence A Haff

Marcel Margulies

John S Shigeura

Brian Fowler

Charles R Connell

Paul G Saviano

Martin Pieprzyk

Geoff Facer

William D Nielsen

Zbigniew T Bryning

Kevin S Bodner

David Eberhart

Ezra Van Gelder

Stephen J Williams

Yuan Li

Eugene F Young

James Ogg

Dean S Burgi

Joseph DiCesare

Mark F Oldham

Linda G Lee

Eric S Nordman

Jens J Hyldig-Nielsen

Dar Bahatt

Jing Wang

Jeffrey M Marmaro

Timothy Z Liu

Alan M Ganz

Richard W Noreiks

Lincoln J McBride

Douglas H Smith

Ralph W Keese

Robert P Raqusa

Reid B Kowalis

Serguei Ermakov

Marcel Margoulies

Ralph Keese

Enrico Picozza

This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.

Sample preparation, processing and analysis systems

The present disclosure relates to methods of identifying target nucleic acids by using coded molecules and its analysis by translocation through a nanopore. Generally, coded molecules are subject to a target polynucleotide dependent modification. The modified coded molecule is detected by isolating the modified coded molecules from the unmodified coded molecules prior to analysis through the nanopore or by detecting a change in the signal pattern of the coded molecule when analyzed through the nanopore.

Methods of detecting target nucleic acids

The present disclosure provides apparatus, systems and method for detecting separately and substantially simultaneously light emissions from a plurality of localized light-emitting analytes. A system according to exemplary embodiments of the present disclosure comprises a sample holder having structures formed thereon for spatially separating and constraining a plurality of light-emitting analytes each having a single nucleic acid molecule or a single nucleic acid polymerizing enzyme, a light source configured to illuminate the sample holder, an optical assembly configured to collect and detect separately and substantially simultaneously light emissions associated with the plurality of light emitting analytes. The system may further include a computer system configured to analyze the light emissions to determine the structures or properties of a target nucleic acid molecule associated with each analyte.

Method and system for multiplex genetic analysis

Embodiments of the present invention provide improved microfluidic devices and related apparatus, systems, and methods. Methods are provided for reducing mixing times during use of microfluidic devices. Microfluidic devices and related methods of manufacturing are provided with increased manufacturing yield rates. Improved apparatus and related systems are provided for supplying controlled pressure to microfluidic devices. Methods and related microfluidic devices are provided for reducing dehydration of microfluidic devices during use. Microfluidic devices and related methods are provided with improved sample to reagent mixture ratio control. Microfluidic devices and systems are provided with improved resistance to compression fixture pressure induced failures. Methods and systems for conducting temperature controlled reactions using microfluidic devices are provided that reduce condensation levels within the microfluidic device. Methods and systems are provided for improved fluorescent imaging of microfluidic devices.

Microfluidic devices and methods

A method for rendering a microfluidic device suitable for reuse for nucleic acid analysis is provided. The method may include flowing a nucleic acid inactivating solution into a microfluidic channel of the device by pumping; and then flowing a wash solution into the channel by pumping, thereby displacing the nucleic acid inactivating solution from the channel, whereby any residual nucleic acid from a prior use of the device is inactivated.

Microfluidic device regeneration

The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more of a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.

Device and method for multiple analyte detection

The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. The device includes a substrate which defines a sample-distribution network having (i) a sample inlet, (ii) one or more detection chambers, and (iii) channel means providing a dead-end fluid connection between each of the chambers and the inlet. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.

Growing community of inventors

Moss Beach, CA, United States of America

Timothy M Woudenberg