Pioneer Hi-bred International, Inc.

E.i. Dupont De Nemours and Company

Corteva Agriscience LLC

Timothy M Nowatzki

Albert Laurence Lu

Scott H Diehn

Christopher Jay Scelonge

Joshua K Young

Cathy Xiaoyan Zhong

James C Register, Iii

Erin L Crowgey

John Lindsey Flexner

M Alejandra Pascual

Douglas S Nubel

Stephen D Thompson

Stephen A Lefko

Rachel R Binning

Bruce H Stanley

Deanne L Wright

Fu-Chih Cheng

Bo Hong

David Onstad

Stephen Thompson

Stephen Lefko

Rachel Binning

James Calvin Register

M Alejandra Villanelo

Iii James Calvin Register

M Alejandra Pascual Villanelo

The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-004114-3 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.

Maize event DP-004114-3 and methods for detection thereof

A method is provided for evaluating a protected plant having a protection mechanism. A population of immature insects (i.e., larvae or nymphs) belonging to an insect species having a plurality of instars is first exposed to the protected plant such that the protected plant is at least contacted thereby. The insect exposure extends for a selected time period corresponding to a sublethal exposure of the insect population to the protected plant. The exposure of the insect population to the protected plant is then halted following the selected time period and insects collected. A physical characteristic of at least some collected insects is measured. The measured physical characteristics are then statistically analyzed so as to determine an efficacy of the protection mechanism of the protected plant with respect to the insect population. Associated methods are also provided.

Method of evaluating plant protection

Laboratory-selected colonies of western corn rootworm exhibiting tolerance to maize containing event DAS-59122-7 are described. Further, methods for various uses of these resistance western corn rootworm colonies are also described, including development of negative cross-resistance strategies and improved resistance management strategies.

Laboratory-selected colonies of western corn rootworm with increased tolerance to maize containing event DAS-59122-7

A method is provided for evaluating a protected plant having a protection mechanism. A population of immature insects (i.e., larvae or nymphs) belonging to an insect species having a plurality of instars is first exposed to the protected plant such that the protected plant is at least contacted thereby. The insect exposure extends for a selected time period corresponding to a sublethal exposure of the insect population to the protected plant. The exposure of the insect population to the protected plant is then halted following the selected time period and insects collected. A physical characteristic of at least some collected insects is measured and the immature insects are then sorted into instars. The measured physical characteristics are then statistically analyzed across the instars so as to determine an efficacy of the protection mechanism of the protected plant with respect to the insect population. Associated methods are also provided.

Growing community of inventors

Granger, IA, United States of America

Timothy M Nowatzki