North Carolina State University

Calabazas Creek Research, Inc.

Timothy Joseph Horn

R Lawrence Ives

David B Marsden

John Christopher Ledford

Rosangela Sozzani

Christopher Dewayne Rock

Provided is a biological specimen holder for positioning multiple specimens for imaging by a light-sheet microscope. The specimen holder allows developing plant embryos, small intact animals, or organs to be imaged in the light-sheet microscope in a single setting. The specimen holders significantly improve the imaging conditions with respect to the standard glass capillary system. Also provided is a semi-automatic image processing pipeline that quantifies cell divisions of plants imaged with both the glass capillary and the novel chambers. Plants imaged using the specimen holder undergo cell divisions for a period at least 16 times longer than those imaged with a glass capillary system and allow increased sample throughput and the option of incorporating light emitting diode (LED) lights to generate a light-controlled environment are also advantages.

Multi-sample chamber for extended term microscope imaging

An RF circuit for an electron beam device is formed with successive layers of fusible metal particles applied in layers and translated with respect to an electron beam. Segments of an electron device may be formed which include an open RF cavity at each end of the segment, which can be machined before the segments are brazed together. In one example, the electron beam device may have iron pole pieces assembled into the partially fused device to provide a magnetic circuit and support for subsequent layers of fused particles. The inner surfaces of the RF circuit structure may be smoothed using an etching or machining operation, and the segments may be brazed together to form a completed RF circuit structure with the magnetic pole pieces included in the monolithic structure segments.

3D printed electron beam device

Growing community of inventors

Raleigh, NC, United States of America

Timothy Joseph Horn