Upfront Chromatography A/s

Upfront Chromatography

Timothy J Hobley

Morten Aae Olander

Marie Bendix Hansen

Allan Otto Lihme

Jurgen Hubbuch

Owen R T Thomas

Allan Otto Fog Lihme

Irini Theodossiou

Owen Robert Tyrynis Thomas

Marcos Simon

The invention relates to a method of distributing a liquid in the fluid bed of an up-flow or a down-flow fluid bed reactor. The invention provides efficient distribution and plug flow like fluid flow through the fluid bed where turbulence and/or back-mixing of the fluid are minimized. In accordance with the invention a fluid bed system for use in treating a fluid by contacting the fluid with a solid phase media is provided and the system includes a reactor chamber adapted to contain the solid phase media and at least one fluid distribution means adapted to distribute and/or deliver the fluid to be treated among the particles of the medium.

Bed adsorption system

The present invention relates to particulate material having a density of at least 2.5 g/ml, where the particles of the particulate material have an average diameter of 5-75 &mgr;m, and the particles of the particulate material are essentially constructed of a polymeric base matrix, e.g. a polysaccharide such as agarose, and a non-porous core material, e.g. steel and titanium, said core material having a density of at least 3.0 g/ml, said polymeric base matrix including pendant groups which are positively charged at pH 4.0 or which are affinity ligands for a bio-molecule. Possible pendant groups include polyethyleneimine (PEI), diethylaminoethyl (DEAE) and quaternary aminoethyl (QAE). The materials are useful in expanded bed or fluidized bed chromatography processes, in particular for purification of bio-macromolecules such as plasmid DNA, chromosomal DNA, RNA, viral DNA, bacteria and viruses.

Particulate material for purification of bio-macromolecules

Growing community of inventors

Copenhagen, Denmark

Timothy J Hobley