Dow Agrosciences LLC

Dowelanco

Wisconsin Alumni Research Foundation

Corteva Agriscience LLC

Doe Agrosciences LLC

Timothy Denver Hey

Kenneth Edwin Narva

Aaron Todd Woosley

Thomas Meade

Justin Michael Lira

Ignacio Mario Larrinua

Donald J Merlo

Joel Jay Sheets

Patricia C Apel-Birkhold

Terence Anthony Walsh

Stephanie L Burton

Sek Yee Tan

Jon Christopher Mitchell

Kristin J Fencil

Sarah E Worden

Sean Michael Russell

Huarong Li

Audrey Jane Etter

Xiaoping Xu

Robin Leola Thompson

Alice E Morgan

Ze-Sheng Li

James A Strickland

Baolong Zhu

Todd P Glancy

Otto Folkerts

Scott Bevan

Kelley Ann Smith

Lining Guo

Krishna M Madduri

Scott B Bintrim

Katherine Armstrong

Qihua C Cai

James K Petell

Janna Mai Armstrong

Thomas A Rocheleau

David J Bowen

Jerald Coleman Ensign

Weiting W Ni

Jean L Roberts

Richard H Ffrench-Constant

Kitisri Sukhapinda

Ted Letherer

Gregory L Orr

Todd A Ciche

Tao Xu

Vimbai Chikwana

Meghan L F Frey

Raymond Fatig

Haley Jo Moon

Amanda D Schleper

Ze Sheng Li

Sue Schoonover

Michael B Blackburn

Terry R Wright

Dayakar Reddy Pareddy

Holly Jean Butler

Navin Elango

Monica Britt Olson

Diaa Alabed

Meghan Frey

Doug A Smith

James Edward King

Premchand Gandra

Gaofeng Lin

Ann Owens Merlo

Ryan M Lee

Nick X Wang

Beth C Rubin-Wilson

Jianquan Li

Carla Ausmus

Tristan E Coram

Nicole Linda Hopkins

Lisa W Baker

Shao-Ching Hung

Xu Xiaoping

Jon C Michell

Elizabeth Caldwell

Haley Ellis

Tonya Strange Moynahan

James E King

Kelley A Smith

The subject invention concerns new classes of insecticidally-active proteins and the polynucleotide sequences which encode these proteins. More specifically, insecticidal proteins of approximately 12-24 kDa and of approximately 12-14 kDa are used for controlling corn rootworms. The subject invention includes methods and transgenic plants for controlling Western Corn Rootworm and other coleopteran insects.

Binary insecticidal Cry toxins

DIG-303 insecticidal toxins, polynucleotides encoding such toxins, use of such toxins to control pests, and transgenic plants that produce such toxins are disclosed.

Dig-303 insecticidal Cry toxins

DIG-305 insecticidal protein toxins, polynucleotides encoding such toxins, and transgenic plants that produce such toxins are disclosed. Also disclosed are methods for using such toxins to control insect pests in plants, especial crop plants. Methods for preparing transgenic plants expressing the protein toxins and methods for detecting the claimed toxins and polynucleotides in transgenic plants are disclosed.

DIG-305 insecticidal Cry toxins

Insecticidal toxins derived from, polynucleotides encoding such toxins, use of such toxins to control plant pests, and transgenic plants that produce, and are protected, by these toxins are described.

IRDIG17912 insecticidal Cry toxins

Engineered and modified Cry6Aa insecticidal toxins, polynucleotides encoding such toxins, use of such toxins to control pests, and transgenic plants that produce such toxins are disclosed.

Engineered Cry6A insecticidal proteins

The subject invention concernsmodified Cry1Ca insecticidal toxins and the polynucleotide sequences which encode these toxins. Uses in transgenic plants are described as are methods for protecting crops from insect pest damage.

Modified Cry1Ca toxins useful for control of insect pests

DIG-17 insecticidal toxins, polynucleotides encoding such toxins, use of such toxins to control pests, and transgenic plants that produce such toxins are disclosed.

DIG-17 insecticidal cry toxins

The subject invention relates in part to Cry3Aa in combination with Cry6Aa. The subject invention relates in part to the surprising discovery that combinations of Cry3Aa and Cry6Aa are useful for preventing development of resistance (to either insecticidal protein system alone) by a corn rootworm (spp.) population. Included within the subject invention are plants producing these insecticidal Cry proteins, which are useful to mitigate concern that a corn rootworm population could develop that would be resistant to either of these insecticidal protein systems alone. The subject invention also relates in part to combinations of Cry3Aa and Cry6Aa proteins 'triple-stacked' or 'multi-stacked' with another insecticidal protein(s) such as a Cry6Aa protein or binary Cry34/35 proteins. Thus, such embodiments target rootworms with three modes of action. Transgenic plants, including corn, comprising a cry6Aa gene and a cry3Aa gene are included within the scope of the subject invention.

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DIG-10 Cry toxins, polynucleotides encoding such toxins, use of such toxins to control pests, and transgenic plants that produce such toxins are disclosed.

DIG-10 insecticidal cry toxins

The present invention includes domain I modifications that improve various attributes of various Coleopteran-active Cry proteins. These attributes can include improved target pest spectrum, potency, and insect resistance management. The subject modifications can affect protoxin activation and the efficiency of pore formation, which can lead to enhanced insect intoxication.

The present invention includes modified, insecticidal B.t. Cry1Ca proteins, including the proteins designated herein as DIG-109 and DIG-152, as well as variants of DIG-109 and DIG-152, nucleic acids encoding these proteins, methods of controlling pests using the proteins, methods of producing the proteins in transgenic host cells, and transgenic plants that produce the proteins. The DIG-109 and DIG-152 proteins comprise chimeric peptides composed of a core toxin segment of B.t. Cry1Ca and a Cry1Ab protoxin segment. Insecticidally active variants of the DIG-109 and DIG-152 proteins are also described.

Modified Cry1Ca insecticial Cry proteins

DIG-5 Cry toxins, polynucleotides encoding such toxins, use of such toxins to control pests, and transgenic plants that produce such toxins are disclosed.

DIG-5 insecticidal Cry toxins

DIG-11 Cry toxins, polynucleotides encoding such toxins, use of such toxins to control pests, and transgenic plants that produce such toxins are disclosed.

DIG-11 insecticidal cry toxins

Growing community of inventors

Zionsville, IN, United States of America

Timothy Denver Hey