Mycogen Plant Science, Inc.

Pioneer Hi-bred International, Inc.

Rhone-poulenc Agro

Lubrizol Genetics, Inc.

Timothy C Hall

Jerry L Slightom

John D Kemp

Dennis W Sutton

Richard F Barker

L Sue Loesch-Fries

Nancy P Jarvis

Jinjiang Dong

Norimoto Murai

Weimin Teng

The present invention provides methods of Agrobacterium-mediated transformation of monocots. The invention further provides methods of making transgenic monocots, as well as transgenic monocots made by the present methods, and seeds and progeny thereof.

Agrobacterium-mediated transformation of monocots

The invention provides genetically modified plant cells having a plant structural gene introduced and expressed therein under the control of a T-DNA promoter. In particular, the invention provides genetically modified plant cells having the phaseolin gene introduced and expressed therein under the control of a T-DNA promoter. Also provided are novel strains of bacteria containing and replicating T-DNA which has been modified to contain an inserted plant structural gene under the control of a T-DNA promoter. Further, the invention provides novel plasmids having the ability to replicate in E. coli and comprising T-DNA which has been modified to contain an inserted plant structural gene under the control of a T-DNA promoter.

Plant structural gene expression

The phaseolin structural gene and promoter are disposed. Also disclosed are plasmids, bacteria, plant cells, plant tissue and plants containing the phaseolin promoter. The promoter finds use in the expression of heterologous plants genes, including the phaseolin gene, in transformed plant cells and plants.

Plant gene expression

The invention provides genetically modified plant cells having a plant structural gene introduced and expressed therein under the control of a T-DNA promoter. In particular, the invention provides genetically modified plant cells having the phaseolin gene introduced and expressed therein under the control of a T-DNA promoter. Also provided are novel strains of bacteria containing and replicating T-DNA which has been modified to contain an inserted plant structural gene under the control of a T-DNA promoter. Further, the invention provides plasmids having the ability to replicate in E. coli and comprising T-DNA which has been modified to contain an inserted plant structural gene under the control of a T-DNA promoter.

A method is disclosed for detecting RNA viral infections in plants comprising the isolation of an RNA molecule, e.g., from alfalfa mosaic virus (AMV), production of full-length cDNA transcripts, cloning into plasmids and propgation of the recombinant plasmid. This recombinant DNA plasmid is utilized diagostically to test a plant for infection by a virus, e.g., AMV.

Growing community of inventors

College Station, TX, United States of America

Timothy C Hall