Karius, Inc.

Timothy A Blauwkamp

Igor D Vilfan

Rene Sit

Fred C Christians

Michael Kertesz

Michael Rosen

Shivkumar Venkatasubrahmanyam

Scott Alexander Smith

Damek Spacek

Liza Huijse

Sivan Bercovici

Lily Blair

Peter J Eugster

David K Hong

Trupti Kawli

Scott Smith

Michael J Rosen

Provided herein are direct-to-library methods, systems, and compositions.

Direct-to-library methods, systems, and compositions

This disclosure provides methods and compositions that are useful for enriching a particular population of nucleic acids (a 'population of interest') within a complex mixture of nucleic acids. The population of interest may make up a minor portion of a complex mixture of nucleic acids. The methods and compositions provided herein are useful for detecting, predicting, diagnosing, or monitoring a disease or disorder, particularly a disease or disorder caused by a foreign microbe or pathogen.

Compositions and methods for enriching populations of nucleic acids

The disclosure provides methods, compositions, systems, and kits for the concurrent detection and analysis of different structural and chemical forms of nucleic acids in a sample.

Sample preparation methods, systems and compositions

This disclosure provides methods for determining relative abundance of one or more non-host species in a sample from a host. Also provided are methods involving addition of known concentrations of synthetic nucleic acids to a sample and performing sequencing assays to identify non-host species such as pathogens. Also provided are methods of tracking samples, tracking reagents, and tracking diversity loss in sequencing assays.

Synthetic nucleic acid spike-ins

This disclosure provides methods, compositions and kits for determining if nucleic acids detected in a sample such as a clinical sample are derived from contaminant pathogens or clinically-relevant pathogens.

Sample series to differentiate target nucleic acids from contaminant nucleic acids

Growing community of inventors

Palo Alto, CA, United States of America

Timothy A Blauwkamp