Aj Innuscreen Gmbh

Ist Innuscreen Gmbh

Other

Aj Cyberton Gesellschaft Fuer Laborautomationssysteme Mbh

Moldiax Gmbh

Timo Hillebrand

Elmara Graser

Thorsten Stroh

Claus Knippschild

Matthias Arndt

Benjamin Jaschinsky

Uwe Wellnitz

Klaus Berka

Volker Hillebrand

Kristin Wessel

Monique Brendel

Biological cells are concentrated and isolated from a sample and/or biological cells are concentrated from a sample, followed by the isolation of nucleic acids from said cells. The sample is brought into contact with a solid phase which has a rough or structured surface.

Process for concentrating cells from a sample and then isolating nucleic acids from said cells

A method includes enriching biomolecules and removal of the biomolecules from a biological sample. In the presence of particles, an alginate solution and salts of divalent and/or polyvalent cations or an acid are added to a biological sample, and an alginate-gel-biomolecule-complex is formed on the particles. The complex is removed from the sample by separation of the particles, and from which subsequently the biomolecules or ingredients of the biomolecules are released. The biomolecules, which shall be enriched, include cell-free nucleic acids, viruses or subcellular microparticles.

Method for enriching biomolecules and for removing the biomolecules from a biological sample

The invention relates to a method for detecting various analytes, characterized by the following steps: a) providing separation particles containing, on their surface, firstly means of binding the analyte to be identified and secondly means of separating the analyte bound to the particles; b) providing identification particles firstly having, on their surface, means for binding the analyte to be identified and secondly containing on their surface or enclosed therein, means which are capable, after they have been detached or released from the particles, by virtue of their labeling, of generating a signal which serves for identification of the analyte; c) combining analyte, separation particles and identification particles; d) removing and washing the identification particles bound via the analyte by means of the separation particles; e) releasing the means which serve to identify the analyte, characterized in that the means which serve to identify the analyte are coupled reversibly to the identification particles and in that the identification molecules serve simultaneously for identification of the analyte and for detection.

Universal method for detecting various analytes

A method and test kit are useful for detecting specific nucleic acid sequences. The process includes (1) matrix-dependent new synthesis of the target nucleic acid; (2) target-specific probe hybridization; and (3) detection of the hybridization event. In the first step, an oligonucleotide 1, which is marked by a marker 1 and is entirely or partially complementary to the target sequence, acts as a primer in the matrix-dependent new synthesis of the target nucleic acid and, in the second step, an oligonucleotide 2, which is marked by a marker 2 and, owing to its melting temperature being lower than that of the oligonucleotide 1, is not involved in the first step, partially or completely hybridizes with the DNA new synthesis product of oligonucleotide 1.

Method for detecting specific nucleic acid sequences

The invention relates to a method for isolation of nucleic acids from aqueous samples containing nucleic acids in free form or liberated by lysis. Before or after the polarity of the aqueous solution is lowered, the sample is brought into contact with a solid phase that has a rough or structured surface, whereupon the nucleic acids precipitate on the solid phase and then, together with the solid phase, are removed from this aqueous solution. The rough or structured surface is preferably a non-smooth metal, plastic or rubber surface. Subject matter of the invention is also a test kit and a corresponding instrument for isolation of nucleic acids.

Method and test kit for rapid isolation of nucleic acids using rough surfaces

A device and method are useful for automated extraction of nucleic acids. The device can include a body that can be immersed partly or completely in a reaction cavity, where the part immersed in the reaction cavity has a non-smooth surface. After lysis, an organic substance, preferably alcohols or ketones, can be mixed with a biological sample. This mixture can be brought into contact with a material with a non-smooth surface. Under these conditions, nucleic acids may be adsorbed on the surface of the material being used. Washing steps may be carried out. After drying, the adsorbed nucleic acid may be detached from the material by adding water or a buffer of low salt concentration, whereupon it can be used for downstream applications.

Device and process for automated extraction of nucleic acids

Device and method for extraction of nucleic acids, comprising a hollow body, preferably a pipette tip, through which a liquid is passed, characterized in that a material with rough or structured surface is disposed in this hollow body in such a way that it can be circumcirculated by a liquid. After lysis of the sample and adjustment of necessary binding conditions for adsorption of the nucleic acids on the carrier material, the mixture, by means of pipetting processes, is repeatedly 'pipetted along' the material for binding nucleic acids, introduced vertically in the pipette tip. The nucleic acids bind to the material. Thereupon washing buffers are likewise 'pipetted along' the material for binding nucleic acids. Then a drying step is performed. Finally, the eluent is again repeatedly 'pipetted along' the vertically disposed material for binding nucleic acids, and in the process the bound nucleic acid is detached. The nucleic acid is now available for necessary downstream application.

Device and method for extracting nucleic acids

The invention relates to a method for size-fractionated isolation of nucleic acids, characterized by the following steps: &#x2014;a first binding buffer, which contains at least one chaotropic salt and at least one substance that raises the pH of the binding buffer, is added&#x2014;in the absence of aliphatic alcohols&#x2014;to a volume of the mixture of nucleic acids, &#x2014;binding on a solid phase and separation of the nucleic acids bound by step a), &#x2014;a second binding buffer, which has a lower pH than the binding buffer under Point a), or a nonionic surfactant or an alcohol or a mixture of nonionic surfactant and alcohol is mixed with the filtrate from step a), &#x2014;binding on a solid phase and separation of the nucleic acids bound by step c), &#x2014;washing and elution, according to known methods, of the nucleic acid isolated after steps a) and c), with the result that the nucleic acids isolated after step a) not only have a larger number of base pairs than the nucleic acids isolated under step c), but also that, both after both step a) and after step c), individual, particular nucleic acid fractions with a particular number of base pairs are isolated that were not isolated in the respective other step. The size ratios of the nucleic acid fractions can be controlled by changing the pH.

Method for the selective size-fractionated separation and isolation of nucleic acid mixtures

A universally usable method for specific detection of target nucleic acid sequences, which method can be performed very rapidly and also simply and furthermore which does not need any expensive instrumental systems. The method is intended to be suitable as a molecular genetic rapid test and to respect the requirements of diagnostic specificity assurance. In this regard it is important that only one specific amplification product be detected and that amplification artifacts can be unambiguously discriminated. A nucleic acid amplification kit suitable for performing this method.

Method and rapid test for the detection of specific nucleic acid sequences

Parallel isolation of a double-stranded nucleic acid and a single-stranded nucleic acid is possible from a sample that contains these acids, without separating the acids, by mixing the sample with a lysis buffer having high salt concentration or low salt concentration, or having a proteolytic enzyme. The sample that contains nucleic acid before its lysis, or the sample that has already been lysed or homogenized, is adjusted with a binding buffer in such a manner that the total nucleic acid is adsorbed onto a solid carrier. The binding buffer contains at least one non-ionic detergent in a high concentration. With the exception of the detergent, the sample contains no other non-acidic organic component miscible in water. The carrier with the adsorbed total nucleic acid is removed. The adsorbed total nucleic acid is washed and eluted.

Method for parallel isolation of viral nucleic acids

The invention relates to a device that allows a target nucleic acid to be detected in a homogeneous batch using two different detection formats. The device comprises at least two heatable sample blocks and a reaction cartridge which contains a base () and at least one film () sealing the base (), wherein the film () comprises a surface () that is not connected to the base () and said surface () forms a volume () for media transfer or at least one reaction chamber (). The device can be used in particular for mobile gene diagnostics under field conditions.

Device for detecting nucleic acids

A mobile rapid test system for nucleic acid analysis. A method comprising the steps of amplification of the nucleic acids by means of rapid-PCR technology, conversion of a double-stranded amplification product into a single-stranded DNA fragment, hybridization with a labeled probe and detection of the nucleic acids on a lateral-flow test strip. A device comprising a reaction cavity which preferably consists of a thin film, inlet and outlet openings for the reaction cavity, one or more heatable sample blocks which are connected to miniaturized cooling bodies and a window for reading off the result. The lateral-flow test strip is a component of the mobile rapid test system. Operation of the instrument system requires no external power source, but only batteries or a rechargeable battery.

Mobile rapid test system for nucleic acid analysis

A method for i) parallel isolation of a double-stranded and/or a single-stranded nucleic acid and/or ii) selective removal of a double-stranded nucleic acid from a mixture of a double-stranded and a single-stranded nucleic acid or from a source comprising a double-stranded and a single-stranded nucleic acid includes absorbing the double-stranded nucleic acid onto a first solid carrier, while the single-stranded nucleic acid is not adsorbed and remains in solution, removing the first carrier with the adsorbed nucleic acid from the solution, mixing the solution comprising the single-stranded nucleic acid with an alcoholic solution having a concentration of 1 to 90 vol.-%, and contacting the resulting solution with second solid carrier, to absorb the single-stranded nucleic acid onto the second solid carrier.

Method for isolating parallel double and single-stranded nucleic acids and for selectively removing double-stranded nucleic acids from a mixture of double and single-stranded nucleic acids

A simple and convenient method for concentrating a biomolecule, including protein or nucleic acid molecules, from a sample. Purified and isolated biomolecules obtained by this method. Methods for improving the specificity or sensitivity of detecting a biomolecule by concentration and/or purification or isolation of the biomolecule according to the method of the invention.

Method for concentrating and isolating biomolecules or viruses

Long- and/or short-chain nucleic acids are separated, purified and recovered by binding the nucleic acid to a solid phase using a binding buffer, to obtain a bonded nucleic acid, and eluting of the bonded nucleic acid from the solid phase, wherein the binding buffer comprises at least one citric acid salt and at least one alcohol.

Method and test kit comprising citric acid salt and alcohol in a binding buffer for the separation, purification and recycling of long- and short-chain nucleic acids

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Hoppegarten, Germany

Timo Hillebrand