Lonza Cologne Gmbh

Lonza Walkersville, Inc.

Octane Biotech Inc.

Timo Gleissner

Andreas Heinze

Ludger Altrogge

Andreas Wirth

Yaling Shi

Eytan Abraham

Herbert Mueller-Hartmann

Timothy Smith

Nuala Trainor

Samatha Bandapalle

Joseph O'Connor

Kelly Purpura

Erika McAfee

Ann Siehoff

Sven Hermsmeier

Thomas Koblizek

Herbert Müller-Hartmann

Joseph O'connor

Disclosed is a method for subjecting adherent cells to at least one electric field, in which the electric field is generated by applying a voltage to at least two active electrodes, wherein at least three electrodesare provided, and wherein at least two electrodesare active electrodeswhen the voltage is applied in order to generate a first electric field, and in which at least one second electric field is generated, wherein at least one of the two previously active electrodesis a potential-free electrodewhen the voltage is applied. Also disclosed is a devicecomprising at least three electrodes, which are connected to at least one voltage source by means of at least one switching device, wherein at least five electrodesare connected to the at least one voltage source by means of four switching devices, wherein at least two electrodesare connected to the voltage source by means of a common switching device. In conjunction with the method described above, the device according to the invention allows the efficient and uniform treatment of adherent cells using an electric field, wherein the switch between active and potential-free electrodes can be carried out quickly and safely using a lower number of switching devices.

Method and device for uniformly treating adherent cells

The present disclosure provides an automated method of producing genetically modified immune cells, including chimeric antigen receptor T (CAR T) cells, utilizing a fully-enclosed cell engineering system.

End-to-end cell therapy automation

Disclosed is a device for applying an electric field to a suspension of cells, comprising at least one chamber which comprises at least one internal space () for holding the suspension, the internal space () comprising at least two segments (), wherein each segment () comprises at least one electrode () and wherein neighboring electrodes () are separated from each other by at least one gap () which is at least partially filled with an insulating material (), and wherein the edges of the electrodes () facing each other within the internal space () are rounded. Rounding the electrodes' edges facing a neighboring electrode results in a significant reduction of field gradients and thus even of the risk of arcing. Also disclosed is a method in which voltage is applied to at least one active electrode () while the electrodes () or electrode segments next and/or opposite to the active electrode () are set to ground potential. Setting neighboring electrodes that surround the active electrode to ground potential results in decreased scattering of the electric field within the internal space so that the electrically active area is locally limited and the field lines are focused near the active electrode and thus control of the process is enhanced.

Device and method for large volume transfection

The invention relates to a device () for applying an electric field to a suspension of cells, cell derivatives, organelles, sub-cellular particles and/or vesicles, comprising at least one chamber () which comprises at least two electrodes (), and at least one separating element () which is movable within the chamber () between two terminal points () and, if it is in a position between the terminal points (), separates at least one first compartment () of the chamber () from at least one second compartment () of the chamber (). According to the invention each compartment () is designed to hold the suspension and comprises at least one port () for charging or discharging the suspension, so as to discharge an aliquot of the suspension from the chamber () and at the same time charge a further aliquot of the suspension into the chamber (), wherein the separating element () is moved in a second direction opposite to a first direction, and wherein the separating element () separates the aliquots from each other.

The invention relates to a device for applying an electric field to a suspension of cells, comprising at least one chamber which comprises at least one internal space () for holding the suspension, the internal space () comprising at least two segments (), wherein each segment () comprises at least one electrode () and wherein neighboring electrodes () are separated from each other by at least one gap () which is at least partially filled with an insulating material (), and wherein the edges of the electrodes () facing each other within the internal space () are rounded. Rounding the electrodes' edges facing a neighboring electrode results in a significant reduction of field gradients and thus even of the risk of arcing. The invention further concerns a method, wherein voltage is applied to at least one active electrode () while the electrodes () or electrode segments next and/or opposite to the active electrode () are set to ground potential. Setting neighboring electrodes that surround the active electrode to ground potential results in decreased scattering of the electric field within the internal space so that the electrically active area is locally limited and the field lines are focused near the active electrode and thus control of the process is enhanced.

The invention relates to a method for subjecting adherent cells to at least one electric field, in which the electric field is generated by applying a voltage to at least two active electrodes, wherein at least three electrodesare provided, and wherein at least two electrodesare active electrodeswhen the voltage is applied in order to generate a first electric field, and in which at least one second electric field is generated, wherein at least one of the two previously active electrodesis a potential-free electrodewhen the voltage is applied. The invention further relates to a devicecomprising at least three electrodes, which are connected to at least one voltage source by means of at least one switching device, wherein at least five electrodesare connected to the at least one voltage source by means of four switching devices, wherein at least two electrodesare connected to the voltage source by means of a common switching device. In conjunction with the method described above, the device according to the invention allows the efficient and uniform treatment of adherent cells using an electric field, wherein the switch between active and potential-free electrodes can be carried out quickly and safely using a lower number of switching devices.

The invention relates to a method for the application of at least one voltage pulse to at least two containers fitted with electrodes, by means of which at least one voltage pulse is applied to at least one container, while at least one other container is subjected to a preparation or a post-processing. According to the invention the method comprises mutual exchange of the respective positions of the container to which a voltage pulse has already been applied and the further container. Furthermore, the invention relates to a device () for making electrical contact with at least one container fitted with electrodes, with at least one receptacle () upon which at least one container can be set, and with at least one contact appliance () for making contact with the electrodes of the container. According to the invention at least two receptacles () are provided, wherein one receptacle () is located at or inside the contact appliance (), and wherein both receptacles () and/or the contact appliance () can be moved such that after completion of the movement the other receptacle () is located at or inside the contact appliance ().

Method and a device for the electrical treatment of a plurality of containers

The invention relates to a method for applying at least one electrical voltage pulse to at least one reaction space by at least partially discharging at least one charge storing unit for storing electrical charges. According to the invention the method comprises applying at least one voltage pulse to a reaction space by at least partially discharging a second charge storing unit and simultaneously charging or partially discharging a first charge storing. Furthermore, the invention relates to a devicefor applying at least one electrical voltage pulse to at least one reaction space, comprising at least two charge storing units () for storing electrical charges and at least one power supply unit () for charging the charge storing units () and/or at least one discharge device for partially discharging the charge storing units (). According to the invention it is provided that at least two of the charge storing units () form a common storage module which can be discharged at any time.

Method and device for the electrical treatment of reaction spaces

The invention relates to a container () with at least three reaction spaces () which in each case have at least one electrode pair for applying an electric voltage for generating an electric field within the reaction space () and which are arranged geometrically in at least one row and/or electrically connected in at least one row, wherein at least one electrode () of a reaction space () is a common electrode () with at least one other reaction space (). According to the invention n+x electrodes () are provided, wherein n is the number of reaction spaces (), with n&#x2267;3 and x is the number of rows, with x&#x2267;1.

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Euskirchen, Germany

Timo Gleissner