Dako Denmark A/s

Agilent Technologies, Inc.

Dakocytomation Denmark A/s

Tim Svenstrup Poulsen

Steen Hauge Matthiesen

Kenneth Heesche Petersen

Lars Winther

Ole Feldballe Rasmussen

Gregory A Testa

Charles M Hansen

Kenneth H Petersen

Kirsten Vang Nielsen

Susan Medom Poulsen

Bent Laursen Ejlertsen

Henning T Mouridsen

Sten Hauge Matthiesen

Bent Laursen Eilertsen

The invention provides methods and compositions for hybridizing at least one molecule to a target. The invention may, for example, eliminate the use of, or reduce the dependence on formamide in hybridization. Compositions for use in the invention include an aqueous composition comprising at least one nucleic acid sequence and at least one polar aprotic solvent in an amount effective to denature double-stranded nucleotide sequences.

Hybridization compositions and methods

The invention provides methods and compositions for hybridizing at least one molecule to a target. The invention may, for example, eliminate the use of or reduce the dependence on formamide in hybridization. Compositions for use in the invention include an aqueous composition comprising at least one nucleic acid sequence and at least one polar aprotic solvent in an amount effective to denature double-stranded nucleotide sequences.

An apparatus () for processing a biological sample (). The biological sample being arranged on a first planar surface () of a carrier (). The apparatus comprises a second planar surface () arranged substantially parallel to said first planar surface and at a first distance from said first planar surface, said first planar surface and said second planar surface are arranged at an angle (A) greater than zero degree from the horizontal plane (HP); supply mean () for supplying an amount () of a liquid that is to be applied to said biological sample. The first planar surface and said second planar surface are configured to be arranged at a second distance from each other, said second distance being such that said supplied amount of liquid is distributed over said biological sample when said first planar surface and said second planar surface are brought to said second distance from each other.

Tissue processing apparatus

An apparatus () for processing a biological sample (). The biological sample being arranged on a first planar surface () of a carrier (). The apparatus comprises a second planar surface () arranged substantially parallel to said first planar surface and at a first distance from said first planar surface, said first planar surface and said second planar surface are arranged at an angle (A) greater than zero degree from the horizontal plane (HP); supply means () for supplying an amount () of a liquid that is to be applied to said biological sample. The first planar surface and second planar surface are configured to be arranged at a second distance from each other, said second distance being such that said supplied amount of liquid is distributed over said biological sample when said first planar surface and said second planar surface are brought to said second distance from each other.

An apparatus () for processing a biological sample (). The biological sample being arranged on a first planar surface () of a carrier (). The apparatus comprises a second planar surface () arranged substantially parallel to said first planar surface and at a first distance from said first planar surface, said first planar surface and said second planar surface are arranged at an angle (A) greater than zero degree from the horizontal plane (HP); supply means () for supplying an amount () of a liquid that is to be applied to said biological sample. The first planar surface and second planar surface are configured to be arranged in a second distance from each other, said second distance being such that said supplied amount of liquid is distributed over said biological sample when said first planar surface and said second planar surface are brought to said second distance from each other.

The present invention provides compositions and methods for the detection of nucleic acid sequences associated with chromosomal aberrations. The invention may, for example, eliminate the use of or reduce the dependence on formamide in hybridization. Compositions for use in the invention include an aqueous composition comprising at least one nucleic acid sequence and at least one polar aprotic solvent in an amount effective to denature double-stranded nucleotide sequences.

Compositions and methods for detection of chromosomal aberrations with novel hybridization buffers

An apparatus for processing a biological sample is provided. The biological sample being arranged on a first planar surface of a carrier, the apparatus having a second planar surface arranged substantially parallel to said first planar surface and at a first distance from said first planar surface. The first planar surface and said second planar surface are arranged at an angle (A) greater than zero degree from the horizontal plane (HP). The apparatus having a supply for supplying an amount of a liquid that is to be applied to said biological sample. The first planar surface and said second planar surface are configured to be arranged at a second distance from each other, said second distance being such that said supplied amount of liquid is distributed over said biological sample when said first planar surface and said second planar surface are brought to said second distance from each other.

An apparatus for processing at least one biological sample accommodated on at least one carrier member () in a chamber includes, at least one reservoir () able to accommodate a fluid on a surface inside the chamber adjacent to and/or facing a substantial part of the at least one biological sample. The apparatus may comprise a bottom member () arranged to support at least one carrier member () carrying at least one biological sample and a lid () including at least one fluid reservoir (). The reservoir filled with water provides humidity to the chamber and impedes drying out of the sample.

Apparatus and method for processing biological samples and a reservoir therefor

An apparatus for processing a biological sample is provided, the biological sample being arranged on a first planar surface of a carrier. The apparatus comprises a second planar surface arranged substantially parallel to said first planar surface and at a first distance from said first planar surface, said first planar surface and said second planar surface being arranged at an angle (A) greater than zero degree from the horizontal plane (HP); and supply means for supplying an amount of a liquid that is to be applied to said biological sample. The first planar surface and said second planar surface are configured to be arranged at a second distance from each other, said second distance being such that said supplied amount of liquid is distributed over said biological sample when said first planar surface and said second planar surface are brought to said second distance from each other.

This invention is directed to compositions and their uses for detection of markers. Such markers may be useful in the understanding of the underlying molecular event leading to a condition or a disease in a subject. These markers may also be useful for characterization of neoplastic cells and cancer cells and their response to certain therapeutical regimes. Therefore the invention as disclosed may contribute to the improvement of the stratification of patients for the best possible treatment.

Compositions and methods for predicting outcome of treatment

Apparatus and methods for processing biological samples and a reservoir therefor

An apparatus for processing at least one biological sample accommodated on at least one carrier member () in a chamber includes at least one reservoir () able to accommodate a fluid arranged on a surface inside the chamber adjacent to and/or facing a substantial part of the at least one biological sample. The apparatus may comprise a bottom member () arranged to support at least one carrier member () carrying at least one biological sample and a lid () including at least one fluid reservoir (). The reservoir filled with water provides humidity to the chamber and impedes drying out of the sample.

The present invention relates to methods for detecting a change in chromosomal structure. These methods employ labeled probes that bind nucleic acids. For example, these probes may be comprised of nucleic acids or nucleic acid analogs and a detectable label.

Growing community of inventors

Horsholm, Denmark

Tim Svenstrup Poulsen