Dow Agrosciences LLC

Sangamo Therapeutics, Inc.

Tim Sawbridge

German Carlos Spangenberg

Steven Robert Webb

Matthew Hayden

Jeffrey C Miller

Dmitry Y Guschin

Manju Gupta

Matthew J Henry

John White Forster

Noel Cogan

W Michael Ainley

John Mason

Joseph F Petolino

William M Ainley

Yidong Ran

Daniel Isenegger

Sandeep Kumar

Stephen N Novak

W Mike Ainley

Disclosed herein are methods and compositions for parallel or sequential transgene stacking in plants to produce plants with selected phenotypes.

Methods and compositions for integration of an exogenous sequence within the genome of plants

A method of gene editing or gene stacking within a FAD3 loci by cleaving, in a site directed manner, a location in a FAD3 gene in a cell, to generate a break in the FAD3 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

FAD3 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks

An Engineered Transgene Integration Platform (ETIP) is described that can be inserted randomly or at targeted locations in plant genomes to facilitate rapid selection and detection of a GOI that is perfectly targeted (both the 5' and 3&#x2032; ends) at the ETIP genomic location. One element in the subject disclosure is the introduction of specific double stranded breaks within the ETIP. In some embodiments, an ETIP is described using zinc finger nuclease binding sites, but may utilize other targeting technologies such as meganucleases, CRISPRs, TALs, or leucine zippers. Also described are compositions of, and methods for producing, transgenic plants wherein the donor or payload DNA expresses one or more products of an exogenous nucleic acid sequence (e.g. protein or RNA) that has been stably-integrated into an ETIP in a plant cell. In embodiments, the ETIP facilitates testing of gene candidates and plant expression vectors from ideation through Development phases.

Engineered transgene integration platform (ETIP) for gene targeting and trait stacking

A method of gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a cell, to generate a break in the FAD2 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

FAD2 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks

Growing community of inventors

Coburg, Australia

Tim Sawbridge