The University of British Columbia

Tim Leaver

Anupam Singhal

Carl Lars Genghis Hansen

Veronique Lecault

Adam Keith White

Oleh Petriv

William Bowden

Dan Da Costa

Leo Wu

Georgia Russell

Darek Sikorski

Michael Vanlnsberghe

Michael VanInsberghe

Carl Lars Hansen

Microfluidic devices are provided for trapping, isolating, and processing single cells. The microfluidic devices include a cell capture chamber having a cell funnel positioned within the cell capture chamber to direct a cell passing through the cell capture chamber towards one or more a cell traps positioned downstream of the funnel to receive a cell flowing. The devices may further include auxiliary chambers integrated with the cell capture chamber for subsequent processing and assaying of the contents of a captured cell. Methods for cell capture and preparation are also provided that include flowing cells through a chamber, funneling the cells towards a cell trap, capturing a predefined number of the cells within the trap, interrupting the flow of cells, flowing a wash solution through the chamber to remove contaminants from the chamber, and sealing the predefined number of cells in the chamber.

Microfluidic cell trap and assay apparatus for high-throughput analysis

Growing community of inventors

Vancouver, Canada

Tim Leaver