Akzo Nobel N.v.

Akzo N.v.

Pamgene Bv

Pamgene International B.v.

Tim Kievits

Peter Franklin Lens

Hermanus Johannes Maria Kreuwel

Bob Van Gemen

Hendrik Sibolt Van Damme

Pieter Jacob Boender

Marinus Gerardus Johannus Van Beuningen

Marinus Gerardus Johannes Van Beuningen

Henriette M Adriaanse

Henriette Maria Adriaanse

Thomas Augustinue Beumer

Marinus Gerardus Van Beuningen

Willem R Boom

The present invention relates to methods for identifying analyte nucleic acids present in a sample comprising nucleic acid molecules, said method comprising the steps of: (a) providing a porous substrate, said substrate comprising a plurality of micro-channels, wherein said micro-channels have immobilized thereon a target molecule capable of binding to an analyte present in said sample; wherein said channels are further provided with analyte amplification components, a reporter system; and said sample; (b) amplifying analyte nucleic acid molecules present within said sample; and (c) allowing binding to take place between an amplified analyte nucleic acid obtained in step (b), said target molecule immobilized onto the channels of said substrate and said reporter system, wherein said reporter system allows detecting whether binding has occurred between said target molecule and said analyte nucleic acid. The present invention further relates to the use of said methods as well as kits for carrying out said methods.

Integrated microarray analysis

Disclosed is a device useful for performing an assay comprising a substrate having interconnecting channels that open out onto a surface for sample application, the channels being provided in at least one cross-sectional area with a first binding substance capable of binding a particular analyte, the substrate being an electrochemically manufactured metal oxide membrane and containing the first binding substance within the through-going channels. Similar devices are also useful for chemical synthesis. Assay and chemical synthesis methods are also disclosed, as are kits for performing the assays or chemical syntheses.

Device for performing an assay, a method for manufacturing said device, and use of a membrane in the manufacture of said device

The present invention is related to an improved method for the quantification of nucleic acid, which can be performed with a minimal amount of nucleic acid amplification reactions. The method according to the invention for the quantification of analyte nucleic acid in a sample comprises the steps of: adding to the sample different respective amounts of different nucleic acid constructs, each construct being distinguishable from the analyte nucleic acid and capable of being co-amplified with the analyte nucleic acid; subjecting the sample to a nucleic acid amplification procedure, using amplification reagents capable of reacting with both the analyte nucleic acid and the nucleic acid constructs; detecting the relative amounts of amplificates derived from analyte nucleic acid and each nucleic acid construct; calculating the amount of analyte nucleic acid from said relative amounts. Each nucleic acid construct is different; the nucleic acid constructs can be distinguished from one another and from the analyte nuclic acid. The nucleic acid constructs do resemble each other, and the analyte nucleic acid, in that all are capable of reacting with the same amplification reagents.

Nucleic acid quantitation by co-amplification of target with multiple

Disclosed is a method of quantifying a target nucleic acid in a test sample by adding to the test sample a known number of molecules of a corresponding nucleic acid comprising a well-defined mutant sequence. Said mutant sequence being discriminatory from the target nucleic acid. Subsequently a competitive amplification reaction of the nucleic acid is performed after which quantification of the amplified nucleic acid is performed by a differential detection.

Quantification of nucleic acid

Elimination of false negatives in nucleic acid detection

The invention is an improved method for the amplification of nucleic acid, wherein ribonucleotides that weaken normal base pairing are introduced during amplification. Preferably, the ribonucleotides are inosine-triphosphate nucleotides which partly substitute guanine-triphosphate nucleotides normally present in the amplification reaction mixture. These ribonucleotides weaken normal base pairing and prevent the formation of secondary structures in the amplificate, thereby increasing the efficiency of amplification. Also, improved sensitivity results during detection of the amplified nucleic acid when the detection method comprises the hybridization of the amplified nucleic acid to a complementary sequence.

Method for nucleic acid amplification using inosine triphosphates to

The present invention is directed to hybridization assays using enzyme-linked oligonucleotide probes, whereby hybridization is detected by separating the hybridized labelled oligonucleotide from the unhybridized probe by gel electrophoresis

Hybridization assays using enzyme-linked probes

The invention relates to a process, a combination of means for isolating nucleic acid from a nucleic acid-containing starting material and a testkit in order to amplify the nucleic acid obtained by said process. More in particular, the invention relates to a process and a kit for isolating nucleic acid from a nucleic acid-containing biological material such as whole blood, blood serum, urine, feces, cell cultures and the like.

Growing community of inventors

Vught, Netherlands

Tim Kievits