Pioneer Hi-bred International, Inc.

E.i. Dupont De Nemours and Company

Centro De Investigacion Y De Estudios Avanzados Del Instituto Politecnico Nacional (cinvestav)

Pioneer Hi-bred, Inc.

Tim Fox

Marc C Albertsen

Mary Trimnell

Gary A Huffman

Yongzhong Wu

Howard P Hershey

Keith S Lowe

Bailin Li

Marianna Faller

Youngzhon Wu

William James Gordon-Kamm

Bo Shen

Mark E Williams

Dale F Loussaert

Jeffrey R Schussler

April L Leonard

Manjit Singh

Brian R Loveland

Juan Pablo San Martin Toloza

Andrew Mark Cigan

Shai J Lawit

Michelle Van Allen

Kristin Haug Collet

Heather Marie Christensen

Jon Aaron Tucker Reinders

Huaxun Ye

Daniel Rodriguez Leal

Nidia Sanchez Leon

Isaac Rodriguez Arevalo

Jaime Padilla Calzada

Gary A Huffma

Marissa Simon

Yongzhon Wu

Sarah Collinson

Rachel Carol Huegel

Jean-Philippe Vielle Calzada

Carlos Barragan Rosillo

Edgar Demesa Arevalo

Carlos Gonzalez Chavez

Elvira Hernandez Lagana

Gloria Leon Martinez

The compositions and methods disclosed relate to DNA compositions, plant cells, seeds, plant parts that relate to maize maintainer plants. Also provided are assays for detecting the presence of the maize DP-056113-9 event based on the DNA sequence of the recombinant DNA construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.

Maize event DP-056113-9 and methods of use thereof

Provided herein are methods and compositions for restoring fertility and maintaining sterility in plants. In particular, one method disclosed includes introducing into a male-sterile plant, wherein the plant comprises one or more homozygous mutations in a male-fertility gene, a plant restoration donor chromosomal component comprising a plant-derived polynucleotide that confers a plant phenotypic marker linked to a male-fertility restoration locus that functionally complements the male-sterility phenotype from the one or more homozygous mutations in the male-sterile plant. In some examples, the plant-derived polynucleotide that confers a plant phenotypic marker and the male-fertility restoration locus are linked to each other and located on the same chromosomal arm on the plant restoration donor chromosomal component. In some examples, the plant-derived polynucleotide that confers a plant phenotypic marker, the male-fertility restoration locus, or both are modified.

Fertility restoration in plants

Methods and compositions useful for avoiding plant meiosis and clonal reproduction through seed are provided herein. The present disclosure provides polynucleotides and related polypeptides of Spo11, Rec8, OSD1-1A, and OSD1-3A and methods and compositions for suppressing their expression level or activity.

Methods and compositions for producing clonal, non-reduced, non-recombined gametes

Compositions and methods are provided for genome modification of a nucleotide sequence located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell or plant to produce a male-sterile plant. In some examples, the methods and compositions employ a guide RNA/Cas endonuclease system for modifying or altering target sites located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell, plant or seed to produce a male-sterile plant. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification a nucleotide sequence located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell to produce a male-sterile plant. Compositions and methods are also provided for restoring fertility to a Ms9, Ms22, Ms26, or Ms45 nucleotide sequence to a male-sterile Ms9, Ms22, Ms26, or Ms45 plant produced using the methods and compositions described herein.

Compositions and methods of modifying a plant genome to produce a MS9, MS22, MS26, or MS45 male-sterile plant

Disclosed are methods of obtaining clonal seeds, methods of plant cloning, methods of screening for maternal plants that produce clonal seeds asexually and methods of increasing yield of clonal seeds. Also disclosed are constructs comprising a nucleic acid that may silence the activity of a RNA-dependent DNA methylation pathway gene. Further disclosed are maternal plants comprising a construct wherein the construct comprises an exogenous nucleic acid sequence, wherein the construct renders the maternal plant defective for RNA-dependent DNA methylation.

Methods for reproducing plants asexually and compositions thereof

Plant cell fate and development is altered by treating cells with cellular reprogramming factors. Embryogenesis inducing morphogenic developmental genes are used as cellular reprogramming factors, specifically comprising polypeptides or polynucleotides encoding gene products for generating doubled haploids or haploid plants from gametes. Maize microspores treated by contacting the isolated cells with an exogenous purified, recombinant embryogenesis inducing morphogenic developmental gene polypeptide results in embryogenesis. The gametes of a maize plant develop into embryoids when transformed with a genetic construct including regulatory elements and structural genes capable of acting in a cascading fashion to alter cellular fate of plant cells. Developmental morphogenic proteins expressed from a genetic construct are used for ex situ treatment methods and for in planta cellular reprogramming.

Systems and methods for cellular reprogramming of a plant cell

A nuclear maize gene is mutated to result in dominant male sterility. Two mutants are provided which impact signal peptide processing. Restoration constructs and methods are provided for use of the dominant male-sterile mutants in producing hybrid seed for male-sterile hybrid plants. Other signal-peptide modifications are provided. Orthologs of the wild type or mutated gene in various species are provided.

Genetic reduction of male fertility in plants

Genetic male sterile plants are provided in which complementing constructs result in suppression of a parental phenotype in the progeny. Methods to generate and maintain such plants and methods of use of said plants, are provided, including use of parental plants to produce sterile plants for hybrid seed production.

Nucleotide sequences of a Msca1 gene, critical to male fertility in plants are described, with DNA molecule and amino acid sequences set forth. Promoter sequences and their essential regions are also identified. The nucleotide sequences are useful in impacting male fertility in plants.

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MSCA1 nucleotide sequences impacting plant male fertility and method of using same

Nucleotide sequences mediating male fertility in plants are described, with DNA molecule and amino acid sequences set forth. Promoter sequences and their essential regions are also identified. The nucleotide sequences are useful in mediating male fertility in plants.

Nucleotide sequences mediating male fertility and method of using same

Growing community of inventors

Des Moines, IA, United States of America

Tim Fox