Roche Sequencing Solutions, Inc.

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Tim Craig

Christos Tzitzilonis

Corissa Harris

Matthew Dipietro

Dhruti Vasudev Dalal

Liv Elisabeth Jensen

Charlotte Yang

Alexander Yang

Cynthia Ann Cech

Cynthia Cech

Dhruti Dalal

Liv Jensen

The present disclosure provides variant OmpG polypeptides, compositions comprising the OmpG variant polypeptides, and methods for using the variant OmpG polypeptides as nanopores for determining the sequence of single stranded nucleic acids. The variant OmpG nanopores reduce the ionic current noise versus the parental OmpG polypeptide from which they are derived and thereby enable sequencing of polynucleotides with single nucleotide resolution. The reduced ionic current noise also provides for the use of these OmpG nanopore variants in other single molecule sensing applications, e.g., protein sequencing.

OmpG variants

OMPG variants

Growing community of inventors

Mountain View, CA, United States of America

Tim Craig