Teknologian Tutkimuskeskus Vtt Oy

Danisco US Inc.

Teknologian Tutkimuskeskus Vtt

Vtt Technical Research Centre of Finland Ltd

Roal Oy

Tiina Pakula

Markku Saloheimo

Merja Elisa Penttila

Ann Westerholm-Parvinen

Mari Hakkinen

Marika Vitikainen

Nina Aro

Michael C Ward

Mari J Valkonen

Anu Koivula

Kaisa Marjamaa

Yun Luo

Felipe Oseas Bendezu

Mari HÄKKINEN

Mari Haekkinen

Merja Penttilae

Merja PenttilÄ

The present invention discloses a polypeptide having beta-glucosidase activity. The activity is retained also in high glucose concentration. The invention also discloses an isolated polynucleotide, a nucleic acid construct and a recombinant host usable in production of said polypeptide, and a method for producing the polypeptide. Further the invention discloses compositions including the polypeptide and method of using the polypeptide in hydrolysis or synthesis.

Beta glucosidase with high glucose tolerance, high thermal stability and broad PH activity spectrum

Certain embodiments of the disclosure are directed to variant filamentous fungal cells, compositions thereof and methods thereof for increased production of one or more proteins of interest. More particularly, in certain embodiments, the disclosure is directed to variant filamentous fungal (host) cells derived from parental filamentous fungal cells, wherein the variant host cells comprise a genetic modification which enables the expression/production of a protein of interest (POI) in the absence of inducing substrate. In certain embodiments, a variant fungal host cell of the disclosure comprises a genetic modification which increases the expression of a variant activator of cellulase expression 3 (ace3) gene encoding an Ace3 protein referred to herein as Ace3-L.

Protein production in filamentous fungal cells in the absence of inducing substrates

The present invention relates to a method for genetically modifying a filamentous fungus host for improved protein production. The method comprises that a filamentous fungus host is genetically modified to overexpress or to be deficient of specific genes. The invention relates also to the modified hosts. Furthermore, the invention relates to a method for improved production or for producing an improved composition of proteins, such as cellulases, hemicellulases, other proteins involved in the degradation of lignocellulosic material, or other proteins, in a filamentous fungus host.

Method for improved protein production in filamentous fungi

Method for protein production in filamentous fungi

The present invention relates to a method for genetically modifying a filamentous fungus host for improved protein production. The method comprises that a filamentous fungus host is genetically modified to be deficient or to overexpress specific genes. The invention relates also to the modified hosts. Furthermore, the invention relates to a method for improved production or for producing an improved composition of proteins, such as cellulases, hemicellulases, other proteins involved in the degradation of lignocellulosic material, or other proteins, in a filamentous fungus host.

Production of proteins in filamentous fungi

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Espoo, Finland

Tiina Pakula