Valtion Teknillinen Tutkimuskeskus

Teknologian Tutkimuskeskus Vtt Oy

Teknologian Tutkimuskeskus Vtt

Panimolaboratorio-Bryggerilaboratorium

Tiina Nakari-Setälä

Merja Penttilä

Markus Linder

Maria-Regina Kula

Johanna Buchert

Richard Fagerström

Hanna Kontkanen

Klaus Selber

Folke Tjerneld

Markku Saloheimo

Laura Ruohonen

Ann Westerholm-Parvinen

Hans Söderlund

Mervi Toivari

Mari Hakkinen

Maija Tenkanen

Marjaana Rättö

Michael Bailey

Kirsi Bromann

Antti Nyyssola

Auli Haikara

Ville Pihlajaniemi

Tuija Kleemola

Pasi Halonen

The present invention concerns a method for producing terpenes in fungi comprising the steps of (a) providing a modified terpene biosynthetic gene cluster inside a host cell, wherein one or more of the naturally occurring genes or promoters of the cluster have been replaced, truncated or removed, (b) providing a transcription factor inside the host cell, the transcription factor activating the terpene biosynthetic gene cluster; (c) cultivating said host in conditions allowing the expression of the transcription factor activating the cluster; and optionally (d) recovering the thus produced terpene product.

Method for producing terpenes

The present invention relates to novel polypeptides, or fragments of polypeptides, genes encoding them and means for producing said polypeptides. In detail the invention relates to polypeptides having esterase, suberinase and/or cutinase activity at low pH. This invention relates also to compositions containing the polypeptides and methods of using the polypeptides.

Polypeptides and active fragments of polypeptides having at least one esterase activity

The present invention relates to polyesterases having cutinase and/or suberinase activity obtainable fromand. The invention further relates to a method for producing the polyesterases, and to polynucleotides, vectors and host cells used therein. The enzymes are useful in hydrolysing cutin, suberin and other polyesters for example in treating agricultural or food raw materials, or wood raw materials, pulp and paper products and waste, and in modifying polyester fibers, or in laundry and dish applications.

Esterases and their use

This invention relates to a method for decreasing the foam formation during cultivation of a microorganism and to a method for producing an enhanced amount of a product of interest. The method comprises that the microorganism is modified in such a way that the microorganism does not produce an essential amount of at least one of the proteins, polypeptides or peptides associated with foam formation during cultivation of the unmodified microorganism. In particular the method comprises that the microorganism is modified not to produce an essential amount of amphipathic or hydrophobic proteins, polypeptides or peptides.

Method for decreasing the foam formation during cultivation of a microorganism

The present invention relates to isolation and purification of protein in aqueous two-phase systems (ATPS). Specifically, the invention provides processes for partitioning of proteins of interest in ATPS by fusing said proteins to targeting proteins which have the ability of carrying said protein into one of the phases.

Process for partitioning of proteins

The present invention relates to immobilization of fusion proteins to surfaces. According to this invention a fusion polypeptide comprises an adhesion polypeptide fused to a preselected polypeptide. Specifically the method takes advantage of the spontaneous immobilization properties of the adhesion polypeptide part of the fusion protein.

Method for immobilization of polypeptides

The present invention relates to isolation and purification of proteins in aqueous two-phase systems (ATPS). Specifically the invention provides processes for partitioning of proteins of interest in ATPS by fusing said proteins to targeting proteins which have the ability of carrying said protein into one of the phases.

The invention relates to a method for determining a gushing factor for a beverage, in which method the quantity of hydrophobin is determined from the raw material of the beverage and/or from the beverage. The hydrophobin determination is performed immunologically using an immunological reaction between a hydrophobin antigen and an antibody.

Method for determining a gushing factor for a beverage

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Espoo, Finland

Tiina Nakari-Setälä