Richter Gedeon Vegyeszeti Gyar Rt

Biogal Gyogyszergyar Rt.

Tibor Klupp

Imre Moravcsik

Lajos Kisfaludy

Andras Balazs

K Alm P Olya

Gy Orgy M At E

Cecilia Egy Ud

Istv An Barta

Eva Guly As

J Anos Sal At

Erika Zilahi

Kornelia Barabas Nee Borbas

Eva Barab As

Istv An Ott

P Eter Kiss

Zsuzsa Bark O N Ee T Oth

Gy Orgy Kiss

K Alm K Oncz Ol

J Anos Erdei

Andr As Patthy

Attila Vincze

Mihaly Sajgo

K Arnly Albrecht

G Abor Ambrus

Istv An Moln Ar

Anik O Tegdes

The present invention relates to a process for producing recombinant desulphatohirudin by means of culturing microorganisms. Concerning the codon usage of microorganisms the synthesized nucleotide sequences were joined downstream of and in reading frame with isolated promoters and signal sequences, subsequently the expression/secretion cassettes comprising the foregoing elements were inserted into plasmid DNAs allowing the cultivation of cells under selective culture conditions. E. coli, Saccharomyces and Streptomyces species were transformed with the said recombinant plasmids to biosynthesize the thrombin inhibitor desulphatohirudin HV-1 which was then isolated and identified. The thus-produced desulphatohirudin can be used to inhibit blood coagulation.

Plasmids and process for producing recombinant desulphatohirudin HV-1

The invention relates to a process for the preparation of a biologically active substance, which can be isolated from normal white blood cells and is capable of inhibiting the proliferation of normal and leukemic myeloid cells selectively. The substance obtained is a peptide, which has an amino acid composition of Tau.sub.1, Asx.sub.1, Ser.sub.2, Thr.sub.1, Glx.sub.3, Gly.sub.2, Ala.sub.1, (PO.sub.4).sub.1.sup.2-, a negative charge at pH 6.5, an electrophoretic mobility of -0.55 to -0.65 related to aspartic acid, has no charge at pH 1.9, has a mobility of about 0.26 related to .epsilon.-DNP-lysine and gives a positive chloro-toluidine reaction.

Process for the preparation of a biologically active substance for

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Budapest, Hungary

Tibor Klupp