Beijing University of Technology

Institute of Biotechnology, Academy of Military Medical Sciences, China

Tiantian Wang

Yi Wang

Bo Liu

Lin Ma

Jing Liu

Min Liu

Li Zhu

Peng Sun

Jun Wu

Hui Tian

Pan Wang

Xin Gong

Chao Pan

Hengliang Wang

Erling Feng

Hongli Suo

Shaohong Chang

Yichen Meng

Mangmang Gao

Yaru Liang

Faxue Peng

The present invention discloses aA with an O-antigen having an extended carbohydrate chain and uses thereof. The method comprises the following steps: inactivating an cld gene encoding an enzyme controlling chain length of O-antigen of aA strain to obtain aA with deletion of cld gene; allowing overexpression of cldgene encoding an enzyme controlling chain length of O-antigen ofinA deficient in the cld gene encoding an enzyme controlling chain length of O-antigen, so as to extend carbohydrate chain length of O-antigen. Both of theA O-polysaccharide-recombinant fusion protein conjugate vaccines rCTB4573-OPSand rEPA4573-OPSas prepared by usingA with an O-antigen having an extended carbohydrate chain can induce mice to generate specific antibodies againstA, and their antibody titers are significantly improved.

with an O-antigen having an extended carbohydrate chain and use thereof

A method for preparing element diffusion-type composite substrate and it belongs to the field of high-temperature coated superconductor substrate preparation. The rolled composite nickel-tungsten alloy substrate is heated and thermally insulated, meanwhile, both ends of the rolled substrate are applied with a low voltage and high current density pulse current. High-performance nickel-tungsten alloy composite substrate is obtained with the method in the present invention and the sandwich-like composite substrate has low ferromagnetism and high strength due to higher solute diffusion from inner layer to outer layer, yet which does not affect the formation of sharp cubic texture on the surface of the composite substrate. On the one hand, the adoption of electric pulse technology accelerates the interdiffusion effect of inter-layer elements, on the other hand, it promotes the recrystallization nucleation and reduces the recrystallization annealing temperature of the composite substrate, thus energy saving effect is achieved and the negative effects of annealing thermal erosion grooves among crystal boundary to subsequent coating are effectively reduced. Alloy composite substrate prepared in this invention has the characteristics of high cubic texture content, low magnetism, high strength, and can be applied to large-scale industrial production.

Method for preparing element diffusion-type composite substrate

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Beijing, China

Tiantian Wang