Enzymaster (Ningbo) Bio-Engineering Co., Ltd.

Tianran Shen

Haibin Chen

Yong Koy Bong

Qing Xu

Zhuhong Yang

Ameng Zhou

Jiadong Yang

Baoqin Cai

Yingxin Zhang

Yaoyao Ji

The present invention provides engineered polypeptides that are useful for the asymmetric synthesis of &#x3b2;-hydroxy-&#x3b1;-amino acids under industrial-relevant conditions. The engineered polypeptides disclosed in this invention were developed through directed evolution based on the ability of catalytic synthesis of (2S, 3R)-2-amino-3-hydroxy-3-(4-nitrophenyl) propanoic acid. The present disclosure also provides polynucleotides encoding engineered polypeptides, host cells capable of expressing engineered polypeptides, and methods of producing &#x3b2;-hydroxy-&#x3b1;-amino acids using engineered polypeptides. Compared to other processes of preparation, the use of the engineered polypeptides of the present invention for the preparation of &#x3b2;-hydroxy-&#x3b1;-amino acids results in high purity of the desired stereoisomers, mild reaction conditions, low pollution and low energy consumption. So, it has good industrial application prospects.

Engineered polypeptides and their applications in the synthesis of beta-hydroxy-alpha-amino acids

The present invention provides engineered polypeptides that are useful for the asymmetric synthesis of &#x3b2;-hydroxy-&#x3b1;-amino acids under industrial-relevant conditions. The present disclosure also provides polynucleotides encoding engineered polypeptides, host cells capable of expressing engineered polypeptides, and methods of producing &#x3b2;-hydroxy-&#x3b1;-amino acids using engineered polypeptides. Compared to other processes of preparation, the use of the engineered polypeptides of the present invention for the preparation of &#x3b2;-hydroxy-&#x3b1;-amino acids results in high purity of the desired stereoisomers, mild reaction conditions, low pollution and low energy consumption. So, it has good industrial application prospects.

Engineered aldolase polypeptides and uses thereof

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Ningbo, China

Tianran Shen