The State University of New York

The Research Foundation of Suny at Stony Brook

Tianbo Liu

Benjamin Chu

Dufei Fang

Dehai Liang

Liguo Song

Yanmei Wang

Qicong Ying

The invention relates to a quasi-interpenetrating network of polymer chains, the chains comprising linear polyacrylamide (LPA) chains in the form of a main frame having a weight average molecular weight of approximately 0.05 million to approximately 25 million g/mole, and a radius of gyration of approximately 10 nm to 350 nm; and polydimethylacrylamide (PDMA) chains prepared by polymerizing PDMA in the presence of the LPA main frame, wherein the LPA and PDMA chains are entangled within one another and interpenetrate one another, and wherein the quasi-interpenetrating network has substantially no chemical cross-linking.

Quasi-interpenetrating networks used as separation media

The present invention is a polymer solution for the efficient separation of charged macromolecules by electrophoresis that includes a plurality of polymers. These polymers are entangled to form an interpenetrating network with greater entanglement times than corresponding homopolymers of the same length. These polymers are different and do not phase separate when dissolved in solution. Preferred polymers are PAM and PVP or PDAM and PVP. The polymer solutions of the present invention provide at least a 500-base read length in one run for a single-stranded DNA separation.

Polymer solution for separation of charged macromolecules by electrophoresis

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Coram, NY, United States of America

Tianbo Liu