Inscripta, Inc.

Tian Tian

Miles Gander

Skylar Stefani

Charles D Johnson

Eileen Spindler

Clint Davis

Patrick Westfall

Andrew Garst

Daniel Held

The present disclosure relates to methods for increasing observed editing rates in the surviving bacteria cells. The compositions and methods presented herein in combination lead to a phenomenon of 'edit or die.' Although less cells survive plating and editing, a large percentage of cells that do survive are multiple editors. In one experiment it was found that if a cell survives transformation, plating, and editing, 75% of the surviving cells are multiple editors; that is, 75% of the surviving cells were simultaneously edited with edits at two or more different locations within the bacterial genome.

Methods for increasing observed editing in bacteria

The present disclosure provides compositions of matter, methods and instruments for editing nucleic acids in live yeast cells.

Simultaneous multiplex genome editing in yeast

The present disclosure provides automated multi-module instrumentation and automated methods for performing recursive editing of live cells with curing of editing vectors from prior rounds of editing.

Curing for recursive nucleic acid-guided cell editing

The present disclosure provides compositions and methods to increase the percentage of edited cells in a cell population when employing nucleic-acid guided editing, as well as automated multi-module instruments for performing these methods. Specifically, the disclosure relates to methods, compositions, modules and automated multi-module cell processing instruments that increase the efficiency of nucleic acid-guided editing in a cell population using a nucleic acid nuclease (i.e., an RNA-guided nuclease or 'RGN')/single-strand binding protein ('SSB') fusion system. The system leverages a single-strand binding protein (SSP) and single-strand DNA annealing protein (&#x201c;SSAP&#x201d;) interactions to drive enhanced recruitment.

Affinity tag for recombination protein recruitment

The present disclosure relates to methods for increasing observed editing rates in the surviving bacteria cells.

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Boulder, CO, United States of America

Tian Tian