Council of Scientific and Industrial Research

Other

Thottapillil Ravindranathan

Uttam Ramrao Kalkote

Rohini Ramesh Joshi

Ramesh Anna Joshi

Sanjay Narayan Nene

Vithal Venkatrao Jogdand

Digambar Vitthal Gokhale

Bhaskar Ganapatrao Gaikwad

Kulbhushan Balwant Bastawde

Shamrao Ganapatrao Patil

Subhash Prataprao Chavan

Digamber Vitthal Gokhale

Kulbhushan Balwant Bastawade

Sandhya Suresh Sudge

Rajkumar B Tejwani

A microbial process for the preparation of D(-)-N-carbamoylphenylglycine from DL-5-phenylhydantoin which comprises culturing the strain pseudomonas sp. having Accession No. NCIM 5070 (ATCC 55940) and deposited in National Collection of Industrial Microorganisms (NCIM), National Chemical Laboratory (NCL) (India), having hydantoinase activity, in a medium for 16-20 hours, separating the cells by centrifugation, treating the solid cells obtained with DL-5-phenylhydantoin in buffer, at a temperature in the range of about 25-30&deg; C. for a period of about 2-6 hours, acidifying the mixture to obtain solid D(-)-N-carbamoylphenylglycine, and separating the product by filtration.

Microbial process for the production of D(-)-N-carbamoylphenylglycine

The present invention relates to a process for the preparation of D(-)N-carbamoylphenylglycine, by growing Pseudomonas sp deposited at National Collection of Industrial Microorganisms, Pune, India and at American Type Cultural Centre, Rockville, U.S.A and afforded accession No. ATCC 55940 in a conventional nutrient, medium, suspending the cells in alkaline buffer containing buffer, acidifying the broth to obtain the product.

Halophilic Pseudomonas strain having accession No.NCIM 5109 (ATCC 55940)

A microbial process for the preparation of D(-)-N-carbamoylphenylglycine from DL-5-phenylhydantoin which comprises culturing the strain pseudomonas sp. having Accession No. NCIM 5070 (ATCC 55940) and deposited in National Collection of Industrial microorganisms (NCIM), National Chemical laboratory (NCL) (India), having hydantoinase activity, in a medium for 16-20 hours, separating the cells by centrifugation, treating the solid cells obtained with DL-5-phenylhydantoin in buffer, at a temperature in the range of about 25-30.degree. C. for a period of about 2-6 hours, acidifying the mixture to obtain solid D(-)-N-carbamoylphenylglycine, and separating the product by filtration.

The invention discloses an improved process for the synthesis of D(+) biotin of the Formula ##STR1## where R.sup.2 represents hydrogen or methyl Biotin is one of the B-complex group of vitamins having immense commercial importance in the area of animal health and nutrition.

Process for synthesis of D(+) biotin

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Pune, India

Thottapillil Ravindranathan