National University of Singapore

Ecole Polytechnique Federale De Lausanne

Thorsten Wohland

Horst Vogel

Ling Chin Hwang

Rudolf H A Rigler

At least two fluorophores (e.g. fluorescein-biotin and tetramethylrhodamine-streptavidin conjugate) for use in fluorescence correlation spectroscopy, characterized in that the fluorophores have substantially the same excitation wavelength (a) and different emission wavelengths (b and d). The fluorophores are used for simultaneous excitation with a single laser wavelength in fluorescence cross-correlation spectroscopy (FCCS). The central wavelength (c) of the dichroic mirror used for the separation of the emission signal lies between the emission wavelengths of the fluorophores.

Fluorescence correlation spectroscopy with single excitation wavelength

The invention concerns an assembly comprising: (a) means for determining the intensity of a plurality of electromagnetic signals, (b) a correlator for correlating or autocorrelating said signals, (c) means for continuously calculating the variance and/or the standard deviation of said signals. It also concerns a method for analysing the intensity of a plurality of electromagnetic radiations scattered and/or emitted from solid, fluid or gaseous samples.

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Singapore, Singapore

Thorsten Wohland