Aj Innuscreen Gmbh

Ist Innuscreen Gmbh

Thorsten Stroh

Timo Hillebrand

The invention relates to a method for isolation of nucleic acids from aqueous samples containing nucleic acids in free form or liberated by lysis. Before or after the polarity of the aqueous solution is lowered, the sample is brought into contact with a solid phase that has a rough or structured surface, whereupon the nucleic acids precipitate on the solid phase and then, together with the solid phase, are removed from this aqueous solution. The rough or structured surface is preferably a non-smooth metal, plastic or rubber surface. Subject matter of the invention is also a test kit and a corresponding instrument for isolation of nucleic acids.

Method and test kit for rapid isolation of nucleic acids using rough surfaces

A device and method are useful for automated extraction of nucleic acids. The device can include a body that can be immersed partly or completely in a reaction cavity, where the part immersed in the reaction cavity has a non-smooth surface. After lysis, an organic substance, preferably alcohols or ketones, can be mixed with a biological sample. This mixture can be brought into contact with a material with a non-smooth surface. Under these conditions, nucleic acids may be adsorbed on the surface of the material being used. Washing steps may be carried out. After drying, the adsorbed nucleic acid may be detached from the material by adding water or a buffer of low salt concentration, whereupon it can be used for downstream applications.

Device and process for automated extraction of nucleic acids

Device and method for extraction of nucleic acids, comprising a hollow body, preferably a pipette tip, through which a liquid is passed, characterized in that a material with rough or structured surface is disposed in this hollow body in such a way that it can be circumcirculated by a liquid. After lysis of the sample and adjustment of necessary binding conditions for adsorption of the nucleic acids on the carrier material, the mixture, by means of pipetting processes, is repeatedly 'pipetted along' the material for binding nucleic acids, introduced vertically in the pipette tip. The nucleic acids bind to the material. Thereupon washing buffers are likewise 'pipetted along' the material for binding nucleic acids. Then a drying step is performed. Finally, the eluent is again repeatedly 'pipetted along' the vertically disposed material for binding nucleic acids, and in the process the bound nucleic acid is detached. The nucleic acid is now available for necessary downstream application.

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Berlin, Germany

Thorsten Stroh