Qiagen Gmbh

Thorsten Singer

Dominic O'Neil

Johann Kubicek

Martin Weber

Sarah Fakih

Maximilian Focke

Holger Wedler

Markus Mueller

Joerg Hucklenbroich

Margit Hiesinger

Corinna Küppers

Nicola Scholle

Sabine Kuchler

Lother Breitkopf

Antje-Katrin Sander

Eva Hänssler

Nicole Seip

Tanya Sperling

Esther Rosenberg

Lothar Breitkopf

Eva HÄNßLER

Karen Kowalewski

The invention inter alia pertains to an electrophoresis assisted method for purifying at least one charged target molecule, preferably a nucleic acid, from a sample. Moreover, a device for use in a method for purifying a charged target molecule by electrophoresis is provided.

Electrophoresis assisted method and device for purifying a charged target molecule from a sample

The present invention provides method for isolating DNA molecules having a size above a certain cut-off value from a DNA containing sample, comprising a) contacting the sample with a binding buffer which comprises a chaotropic agent and a buffering agent to provide a binding mixture and binding DNA molecules having a size above the cut-off value to a binding matrix which has a silicon containing surface, wherein the cut-off value is determined by the pH value of the binding mixture; b) separating the bound DNA from the remaining sample; c) optionally washing the bound DNA; and d) optionally eluting the bound DNA from the binding matrix. Said method allows the size selective purification of DNA molecules.

Method for separating DNA by size

Provided is inter alia to an electrophoresis assisted method for purifying a target nucleic acid from a nucleic acid containing sample, comprising The method is particularly suitable for isolating RNA. The liquid medium delays elution of the RNA from the solid phase, thereby preventing a degradation of the RNA by e.g. RNases.

Electrophoresis assisted method for purifying a target nucleic acid using a delayed elution approach

The present invention relates to a method for selectively depleting animal nucleic acids from non-animal nucleic acids in a sample, which comprises animal cells and at least one further type of cells, selected from microbial cells and plant cells or a combination thereof, to a lysis solution A to be used in and to a kit to carry out said method as well as to the use of a particular silica membrane as both, a filtration matrix for separating essentially intact microbial and/or plant cells from lysed animal cells and an adsorption matrix for nucleic acids, in particular in a method according to the present invention.

Method, lysis solution and kit for selectively depleting animal nucleic acids in a sample

Provided is a method for extracting nucleic acids from a wax-embedded sample, and use of particular solvents for removing wax from a wax-embedded sample for extracting, isolating and/or purifying nucleic acids from a crosslinked wax-embedded sample.

Extraction of nucleic acids from wax-embedded samples

The invention relates to a chromatographic device for isolating and/or purifying double-stranded nucleic acids, preferably double-stranded DNA, from a mixture of such nucleic acids with single-stranded nucleic acids, oligonucleotides, mononucleotides, salts and/or other such impurities. The invention also relates to a method for chromatographically isolating and/or purifying same, and to a kit for this purpose.

Method and device for isolating and purifying double-stranded nucleic acids

The present invention relates to an apparatus and a method for the more efficient cleaning of nucleic acids, and to a kit for carrying out this method.

Apparatus and method for the more efficient isolation of nucleic acids

The present invention relates to a method for cleaning and isolating nucleic acids using cationic detergents with the general formula (I):YRRRRX&#x2003;&#x2003;(I)where

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Hilden, Germany

Thorsten Singer