Upfront Chromatography A/s

Thorkild Christian Bo Slashedg Hansen

Allan Otto Lihme

Claus Schafer Nielsen

A fluidized bed chromatographic process for purification and binding of molecules in a liquid by binding the molecules to an active substance covalently bound to chromatographic adsorbent particles in which process a fluidized bed of the adsorbent particles is formed and said liquid is passed through the fluidized bed, wherein the chromatographic adsorbent particles are formed of a porous composite material having pores allowing access to the interior of the composite material by the molecules, and the porous composite material includes: (i) density controlling particles which are either (a) hollow low density particles which are impermeable to the liquid and have a density providing floatation of the adsorbent particles in the liquid or, (b) high density particles having a density providing sedimentation of the adsorbent particles in the liquid; (ii) a matrix formed by consolidating at least one conglomerating agent selected from the group consisting of natural and synthetic organic monomers and polymers; and (iii) an active substance for binding molecules in the liquid; the density controlling particles being embedded in the matrix, and the active substance being covalently bound to the matrix; wherein; the adsorbent particles have a relative density with respect to the liquid which is less than 0.95 or greater than 1.1 and a particle size within the range of 50-750 .mu.m, and each of the relative density and particle size range of the adsorbent particles is selected to provide desired floatation/sedimentation properties of the adsorbent particles in the liquid in the fluidized bed process with substantially no turbulence.

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Hellerup, Denmark

Thorkild Christian Bo Slashedg Hansen