Lonza Inc.

Lonza Ag

Other

Thomas Zimmermann

Karen T Robins

Walter Brieden

Andrew Brian Naughton

Nicholas Shaw

Andreas Tinschert

Andreas Kiener

Knut Burgdorf

Catherine Caubere

Cristiana Boraschi

Shigeaki Harayama

Elisabeth Bohlen

Olwen M Birch

Josef Werlen

The present invention relates to a novel process for the preparation of (S)&#x2014; or (R)-3,3,3-trifluoro-2-hydroxy-2-methylpropionic acid and to novel microorganisms capable of utilizing the propionamide of the formula in the form of the racemate or of its optically active isomers as the sole nitrogen source.

Process for the preparation of (S)- or (R)-3,3,3-trifluoro-2-hydroxy-2-methylpropionic acid

The present invention relates to a novel process for the preparation of (S)- or (R)-3,3,3-trifluoro-2-hydroxy-2-methylpropionic acid and to novel microorganisms capable of utilizing the propionamide of the formula in the form of the racemate or of its optically active isomers as the sole nitrogen source.

Process for the preparation of (S) - or (R) -3,3,3-trifluoro-2-hydroxy-2-methylpropionic acid

Described are new micro-organisms and a new enzyme capable of using as sole source of nitrogen the propionic acid amide of formula (VI), in racemate form or as optically active isomers. Described also is a method of preparing (S)- or (R)-3,3,2-trifluoro-2-hydroxy-2-methylpropionic acid of formulas (I) and (II) starting from trifluoroaceto-acetic ester. The first three process steps are chemical, the fourth process step microbiological.

Method of preparing (S)- or (R) -3,3,3-trifluoro-2-hydroxy-2-methylpropionic acid

New microorganisms with plasmids stable relative to betaine use. These new microorganisms contain (a) a hybrid plasmid with a DNA fragment that contains a genetic sequence that codes for the use of betaine and (b) a mutation in the chromosomal gene coding for the betaine use.

Microorganisms for the stabilization of plasmids

New microorganisms with plasmids stable relative to betaine utilization. These new microorganisms contain (a) a hybrid plasmid with a DNA fragment that contains a genetic sequence that codes for betaine utilization and (b) a mutation in the chromosomal gene coding for the betaine utilization.

Genes for butyrobetaine/crotonobetaine-l-carnitine metabolism and their

A genetic engineering process for the production of S-(+)-2,2-dimethylcyclopropanecarboxamide. For this purpose, a new DNA is isolated from a microorganism that codes for a stereospecific hydrolase. This DNA is then ligated in an expression vector, and a hybrid plasmid results, which is transformed into microorganisms. These microorganisms are then able to biotransform the R-(-) isomer in racemic R,S-(.+-.)-2,2-dimethylcyclopropanecarboxamide into R-(-)-2,2-dimethylcyclopropanecarboxylic acid, and optionally active S-(+)-2,2-dimethylcyclopropanecarboxamide is obtained.

Genetic engineering process for the production of

A microbiological process for terminal hydroxylation of ethyl groups on aromatic 5- or 6-member ring heterocycles. The hydroxylation is performed with microorganisms which:

Process for terminal hydroxylation of ethyl groups on aromatic 5- or

A process using microorganisms which contain genes, which form an active xylene monooxygenase, which form no effective, chromosomally or plasmid-coded alcohol hydrogenase, and which are, thus, capable of hydroxylating methyl groups on aromatic 5- or 6-atom heterocycles to the corresponding hydroxymethyl derivatives, for the production of hydroxymethylated 5- or 6-atom heterocycles.

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Naters, Switzerland

Thomas Zimmermann