Basf Aktiengesellschaft

Basf Se Corporation

Thomas Zelinski

Bernhard Hauer

Maria Keβeler

Andreas Schmid

Juergen Gert Tropsch

Thomas Pfeiffer

Thomas Friedrich

Irene Troetsch-Schaller

Jürgen Tropsch

Frank-Olaf Maehling

Frank-Olaf Mähling

Wolfgang Kasel

Uwe Rebholz

Irene Trötsch-Schaller

Maria Kesseler

Bruno Bühler

Bernhard Witholt

Frank Hollmann

Christiane Karla Steckhan, Legal Representative

Uwe Steckhan, Legal Representative

Antje Steckhan, Legal Representative

Heike Steckhan, Legal Representative

The present invention relates to methods for expressing nucleic acid sequences in prokaryotic host cells, where at least one DNA construct which is capable of episomal replication in a host cell and which comprises a nucleic acid sequence to be expressed under the transcriptional control of an L-rhamnose-inducible promoter, where the promoter is heterologous with regard to the nucleic acid sequence, is introduced into the host cell and the expression of he nucleic acid sequence is induced by addition of L-rhamnose, wherein the prokaryotic host cell is at least deficient with regard to an L-rhamnose isomerase.

L-rhamnose-inducible expression systems

The invention relates to a method for preserving and/or storing microorganisms which exhibit at least one nitrile hydratase or nitrilase enzyme activity, with the preservation and/or storage being effected in an aqueous medium which comprises at least one aldehyde, with the total aldehyde concentration being in a range from 0.1 to 100 mM/l.

Methods for preserving and/or storing cells having a nitrilase or nitrile hydratase activity

The invention relates to the use of polymers which comprise, in copolymerized form, an &#x3b1;-olefin, at least one alkenyl ester and at least one ester of an &#x3b1;,&#x3b2;-unsaturated carboxylic acid with higher alcohols as an additive for fuel oils and lubricants and especially as a cold flow improver in fuel oils; to the fuel oils and lubricants additized with these polymers; and to additive packages comprising such copolymers. The invention also relates to quaternary and higher copolymers which comprise the abovementioned monomers in copolymerized form.

Cold flow improver

Process for the continuous preparation of ethylene copolymers by free-radical copolymerization of ethylene and at least one comonomer in a cascade comprising at least two reactors, wherein one or more initiators are fed into the reaction mixture downstream of the first reactor.

Method for producing ethylene copolymers

The present Invention relates to new nitrilases with modified Substrate acceptance, the method to obtain them and the use of the aforesaid nitrilases. Said nitrilases are encoded by a polypeptide sequence, which at a Position corresponding to Position 296 in wild-typecomprises an amino acid which is not tyrosine. The Invention further relates to nucleic acid sequences and amino acid sequences encoding Said nitrilases, expression constructs comprising Said nucleic acid sequences, vectors comprising the nucleic acid sequences or the exPression constructs, to organisms, preferably microorganisms, comprising the nucleic acid sequences, expression constructs or vectors. The Invention additionally relates to a method for preparing carboxylic acids, preferably substituted chiral carboxylic acids from racemic nitriles.

Modified nitrilases and their use in methods for the production of carboxylic acids

The present invention relates to alkyl ether sulfate salts of the general formula IRO&#x2014;(CHCHO)&#x2014;(CH&#x2014;CHRO)&#x2014;(CHCHO)SOM&#x2003;&#x2003;(I),where and to their use as anion surfactant component in laundry detergents and cleaning compositions, in chemical engineering applications or in cosmetics formulations.

Alkyl ether sulfates

The invention relates to a process for the biocatalytic oxidation of aromatic compounds using recombinant xylene monooxygenase-expressing microorganisms in a biphasic reaction medium.

Method for the oxidation of aromatic compounds

A method comprising the indirect electrochemical regeneration of NAD(P)H in enzymatic substrate conversions which are catalyzed by monooxygenases, for example, is described. In particular, a method for the electroenzymatic preparation of 2,3-dihydroxyphenyl derivatives, which is catalyzed by the enzyme 2-hydroxybiphenyl 3-monooxygenase and in which simultaneously the NADformed by reductive cleavage of oxygen is electrochemically reduced, is described.

Method comprising the indirect electrochemical regeneration of NAD(P)H

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Neuleiningen, Germany

Thomas Zelinski