Lucigen Corporation

Thomas W Schoenfeld

David Mead

Ronald Godiska

Robert A Difrancesco

Spencer Hermanson

Vinay K Dhodda

David A Mead

Thermostable viral polymerases exhibiting a combination of activities selected from, proofreading (3'-5&#x2032;) exonuclease activity, nick translating (5&#x2032;-3&#x2032;) nuclease activity, synthetic primer-initiated polymerase activity, nick-initiated polymerase activity, reverse transcriptase activity, strand displacement activity, and/or decreased discrimination against incorporation of nucleotide analogs. Also provided are compositions including the polymerases, polynucleotides encoding the polymerases and methods of using the polymerases.

Thermostable viral polymerases and methods of use

Vector preparations and cloning constructs suitable for use in cloning are provided. Vector preparations are double-stranded DNA molecules having two 3' termini, each terminus having a single base pair overhang that is capable of hybridizing to a single base pair overhang on a double stranded polynucleotide sequence to be cloned. The overhang of the vector preparation is suitably a dCMP and the overhang of the polynucleotide sequence to be cloned is suitably a dGMP. In other embodiments, the overhang of the polynucleotide sequence to be cloned is any ddNTP and the corresponding overhang of the vector preparation is any base that pairs to the ddNTP. The latter embodiment is particularly suited to preparing recombinant molecules having only a single insert. Methods of cloning, methods of preparing libraries of recombinant molecules and kits for carrying out the methods are also provided.

Vectors, kits and methods for cloning DNA

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Madison, WI, United States of America

Thomas W Schoenfeld