Cargill Inc.

Bioamber Inc.

Thomas W McMullin

Brian Jeffrey Rush

Gregory Michael Poynter

Arlene M Fosmer

Kevin T Watts

Vernon L McIntosh, Jr

Kenneth R Finley

Aristos Aristidou

Catherine Asleson Dundon

Kari Koivuranta

Beth M Mastel

Jeanette M Huryta

Benjamin Matthew Hause

Kevin Roberg-Perez

Keith M Brady

Holly J Jessen

Pirkko Suominen

Ana Negrete-Raymond

Erin Kathleen Marasco

Amit Vas

Pirrko Suominen

Yeast cells having a reductive TCA pathway from pyruvate or phosphoenolpyruvate to succinate, and which include at least one exogenous gene overexpressing an enzyme in that pathway, further contain an exogenous transhydrogenase gene.

Yeast cells having reductive TCA pathway from pyruvate to succinate and overexpressing an exogenous NAD(P+) transhydrogenase enzyme

Less-than-diploidcells are produced. The cells have at least one unpaired chromosome and may be haploid, i.e., are missing one member of each pair of chromosomes that are present in the wild-type strains. The less-than-diploid cells are useful fermentation strains, performing similarly to diploid strains that are otherwise similarly engineered. The less-than-diploid strains can be mated to produce diploids, which themselves are useful fermentation strains. The less-than-diploid strains are also useful as host strains for producing further genetically modified strains that can be less-than-diploid or mated to produce diploids.

Genetically modified haploid

Yeast cells are genetically modified to disrupt a native metabolic pathway from dihydroxyacetone to glycerol. In certain aspects, the yeast cell is of the generaor. In other aspects, the yeast cell is capable of producing at least one organic acid, such as lactate. The yeast cells produce significantly less glycerol than the wild-type strains, and usually produce greater yields of desired fermentation products. Yeast cells of the invention often grow well when cultivated, despite their curtailed glycerol production.

Yeast cells having disrupted pathway from dihydroxyacetone phosphate to glycerol

The present application provides genetically modified yeast cell comprising an active succinate fermentation pathway, as well as methods of using these cells to produce succinate.

Compositions and methods for succinate production

Yeast cells having reductive TCA pathway from pyruvate to succinate and overexpressing an exogenous NAD(P)+ transhydrogenase enzyme

Fermentation process using yeast cells having disrupted pathway from dihydroxyacetone phosphate to glycerol

Recombinant yeast cells contain a reductive TCA pathway from phosphoenolpyruvate or pyruvate to succinate. At least one metabolic step in the pathway includes a reaction of NADPH to produce NADP+. The yeast cell contains at least one exogenous NADPH-dependent gene in the pathway from phosphoenolpyruvate or pyruvate to succinate, preferably an NADPH-dependent malate dehydrogensase or fumarate reducase gene (or both).

Yeast cells having NADP(H)-dependent reductive TCA pathway from pyruvate to succinate

Growing community of inventors

Minnetonka, MN, United States of America

Thomas W McMullin