Northwestern University

Thomas V O'Halloran

Teresa K Woodruff

Emily L Que

Alison M Kim

Betty Kong

Miranda L Bernhardt

Denana U Miodragovic

Masayasu Taki

SonBinh T Nguyen

Seth A Garwin

Haimei Chen

Stephen P Watton

Frederick M MacDonnell

Nathanael L Fackler

Sang-Min Lee

The invention relates generally to compositions and methods for the detection of zinc. Provided herein is a class of zinc-responsive probes with tunable photophysical properties that can be modified for coupling to a solid support or other chemical moieties. In particular, modifications to the 5-position of the BODIPY core allows for alteration of probe properties and functionalities.

Zinc-responsive fluorophores

The invention relates generally to compositions and methods for the detection of zinc. In particular, compositions and methods are provided to detect changes in cellular zinc concentration and to correlate them to cellular phenomena.

Compositions and methods for the detection of zinc

Disclosed herein are methods of treating a cancer cell that include contacting a cancer cell with a compound having the structure of formula (I) M is Pt, Pd or Ni; Q is As, Sb or Bi; Zis N; Zis O or S; Land Lare independently C(O), C&#x2014;Ror C&#x2014;R; X is a Lewis base; Yand Yare independently selected from &#x2014;OR, &#x2014;OR, &#x2014;SRand &#x2014;SR, wherein Rand Rare independently selected from hydrogen, halogen, cyano, keto, ester, ether, thiol, thioether, thioester, imino, C-Calkyl, C-Calkenyl, alkynyl, alkoxyl, amino, amidyl, immino, sulfonyl, sulfoxyl, phosphoryl, phosphoryl ester, glycosyl, aryl, C-Ccycloalkyl, heteroaryl, and C-Cheterocycloalkyl; and Rand Rare independently selected from hydrogen, C-Calkyl, C-Calkenyl, alkynyl, alkoxyl, amino, amidyl, immino, sulfonyl, sulfoxyl, phosphoryl, phosphoryl ester, glycosyl, aryl, C-Ccycloalkyl, heteroaryl, and C-Cheterocycloalkyl.

Arsenoplatin anti-cancer agents

Disclosed herein are conjugates that include a ligand, and a compound having the structure of formula (I) M is Pt, Pd or Ni; Q is As, Sb or Bi; Zis N; Zis O or S; Land Lare independently C(O), C&#x2014;Ror C&#x2014;R; X is a Lewis base; Yand Yare independently selected from &#x2014;OR, &#x2014;OR, &#x2014;SRand &#x2014;SR, wherein Rand Rare independently selected from hydrogen, halogen, cyano, keto, ester, ether, thiol, thioether, thioester, imino, C-Calkyl, C-Calkenyl, alkynyl, alkoxyl, amino, amidyl, immino, sulfonyl, sulfoxyl, phosphoryl, phosphoryl ester, glycosyl, aryl, C-Ccycloalkyl, heteroaryl, and C-Cheterocycloalkyl; and Rand Rare independently selected from hydrogen, C-Calkyl, C-Calkenyl, alkynyl, alkoxyl, amino, amidyl, immino, sulfonyl, sulfoxyl, phosphoryl, phosphoryl ester, glycosyl, aryl, C-Ccycloalkyl, heteroaryl, and C-Cheterocycloalkyl.

Disclosed are compound having the structure of formula (I): wherein M is Pt, Pd or Ni; Q is As, Sb or Bi; Zis N; Zis O or S; Land Lare independently C(O), C&#x2014;Ror C&#x2014;R; X is a Lewis base and Yand Yare independently oxygen- or sulfur-containing substituents, conjugates containing compounds of formula (I) and their use as chemotherapy agents.

The present invention provides liposomal compositions and methods of using such compositions in vitro and in vivo. In particular, the present invention provides stable, polymer-caged liposomes comprising a pH responsive delivery mechanism for delivery of nucleic acids, peptides, small molecules, drugs, etc. in vitro and in vivo.

Compositions and methods for polymer-caged liposomes

The present invention provides methods, compositions, and systems for reinitiating meiosis in cells in meiotic arrest and oocyte activation in fertilized, but un-activated, oocytes. In certain embodiments, Zn-binding moieties (e.g., zinc chelators) are used for reinitiating meiosis or oocyte activation.

Modulation of oocyte meiotic progression and oocyte activation

The present invention relates to methods and compositions for the treatment of cancer and for research and analysis of cancer. In particular, the present invention provides encapsulated arsenic anti-cancer compositions with improved properties.

Encapsulated arsenic drugs

Benzoxazole fluorescent sensor compounds and related ratiometric imaging methods for zinc metal ion.

Zinc-chelating ratiometric fluorescent probes and related methods

The present invention provides a new tetradentate bis-amido bis-alkoxo, 1,2-(bis-2,20diphenyl-2-hydroxyethanamido)benzene (H.sub.4 PHAB) and various derivatives thereof. This ligand is able to stabilize high valen metal oxo complexes by providing strong sigma donor ligands and through the use of steric bulk to prevent decomposition via formation of the .mu.-oxo dimers. Disclosed are novel metal oxo structural chemistry and oxidation reactions carried out with the novel metal oxo complexes.

High oxidation state metal oxo complexes of the PHAB ligand

Disclosed herein is a bi-metallic cross-linking reagent according to the following formula: ##STR1## where M.sub.1 is a metal ion species capable of forming a complex of coordination number four or coordination number six; wherein L.sub.1, L.sub.2, L.sub.3, and L.sub.4 are each independently a halide, ammonia, dimethyl sulfoxide, carboxylate, thiolate, imidazole, a nucleobase, or an empty coordination site, provided that no more than two of L.sub.1, L.sub.2, L.sub.3, and L.sub.4 are empty coordination sites; wherein M.sub.2 is a metal ion species capable of forming a complex of coordination number two with a first ligand that is a hydrocarbon moiety and a second ligand that is kinetically lablie; and wherein n is an integer from two to nine. Also disclosed are cross-linked species, methods of preparing the crosslinking reagents of the invention, and methods for their use in tissue-specific targeting of anti-tumor agents.

Growing community of inventors

Chicago, IL, United States of America

Thomas V O'Halloran