Bristol-myers Squibb Compnay

Thomas Spires

Marco Gottardis

Matthew V Lorenzi

Roberto Weinmann

Dan You

Methods for determining whether a test agent inhibits a 17&#x3b2;-HSD3, the methods comprising: obtaining a recombinant host cell that expresses the 17&#x3b2;-HSD3; obtaining a reaction mixture comprising the 17&#x3b2;-HSD3, androstenedione, and the test agent; measuring the amount of testosterone in the reaction mixture by a scintillation proximity assay (SPA) using SPA beads conjugated with a testosterone-specific antibody, wherein when the amount of testosterone is lower in the presence of a test agent than in the absence of the test agent, the test agent is identified as an inhibitor of the 17&#x3b2;-HSD3.

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Monroe, NJ, United States of America

Thomas Spires