Perkinelmer Cellular Technologies Germany Gmbh

Evotec Technologies Gmbh

Evotec Biosystems Ag

Other

Stmicroelectronics S.r.l.

Fraunhofer-gesellschaft Zur Foerderung Der Angewandten Forschung E.v.

Institut Pasteur

Nmi Naturwissenschaftliches Und Medizinisches Institut an Der Universitat Tubingen

Thomas Schnelle

Rolf Hagedorn

Torsten Müller

G&uuml;nter Fuhr

Torsten M&uuml;ller

Gunter Fuhr

Gabriele Gradl

Günter R Fuhr

Stefan Fiedler

Stephen Graham Shirley

Henning Glasser

Christoph Reichle

Torsten Mueller

Guenter R Fuhr

Bernd Wagner

Martin Stelzle

Andreas Voigt

Mario Giovanni Scurati

Jan Gimsa

Jan Hornung

Stephen Shirley

Spencer Shorte

Shamoi Monajembashi

Karl-Otto Greulich

Hermine Hitzler

G&uuml;nter F&uuml;hr

Frank Grom

Jorg Kentsch

Daniel Mietchen

Jörg Kentsch

Methods and devices for the separation of particles () in a compartment () of a fluidic microsystem () are described, in which the movement of a liquid () in which particles () are suspended with a predetermined direction of flow through the compartment (), and the generation of a deflecting potential in which at least a part of the particles () is moved relative to the liquid in a direction of deflection are envisaged, whereby further at least one focusing potential is generated, so that at least a part of the particles is moved opposite to the direction of deflection relative to the liquid by dielectrophoresis under the effect of high-frequency electrical fields, and guiding of particles with different electrical, magnetic or geometric properties into different flow areas () in the liquid takes place.

Methods and devices for separating particles in a liquid flow

The invention relates to an operating method for a microfluidic system, including the following steps: feeding of a carrier flow with particles () of a first particle type suspended therein into the microfluidic system; charging of a plurality of electrical field cages () in the microfluidic system with the supplied particles () of the first particle type; the supplying of a carrier flow with particles () of a second particle type suspended therein into the microfluidic system; and charging the field cages () in the microfluidic system with the supplied particles () of the second particle type in such a manner that a particle () of the first particle type and a particle () of the second particle type is present in at least one of the field cages (). The invention also relates to a corresponding microfluidic system.

Microfluidic system and corresponding operating method

A plurality of planar electrodes () in a microchannel () is used for separation, lysis and PCR in a chip (). Cells from a sample are brought to the electrodes (). Depending on sample properties, phase pattern, frequency and voltage of the electrodes and flow velocity are chosen to trap target cells () using DEP, whereas the majority of unwanted cells () flushes through. After separation the target cell () are lysed while still trapped. Lysis is carried out by applying RF pulses and/or thermally so as to change the dielectric properties of the trapped cells. After lysis, the target cells () are amplified within the microchannel (), so as to obtain separation, lysis and PCR on same chip ().

Treatment of biological samples using dielectrophoresis

A description is given of methods for collecting particles () which are suspended in a liquid, including the following steps: providing the liquid containing the suspended particles () in a compartment () having lateral surfaces (), wherein at least one electrode () is arranged on at least one of the lateral surfaces (), and generating high-frequency electric fields by means of the at least one electrode () so as to form at least one circulating flow (), by means of which the particles () are guided to at least one predetermined collecting area () in the compartment (), wherein the flow () is formed in such a way that at least one branch of the flow runs along a longitudinal extent of the at least one electrode (), and the flow () circulates about an axis () which is oriented perpendicular to the respectively adjacent lateral surface () with the electrode (). Corresponding devices for collecting particles are also described.

Method and device for collecting suspended particles

A method for high-resolution image recording of at least one object with a microscope, includes the steps of: (a) positioning the object in a receptacle being arranged in the optical axis of the microscope, (b) generating at least two first data sets per object which represent intermediate images of the object with at least two different orientations relative to the optical axis of the microscope, wherein the different orientations of the object are provided by moving the object relative to the receptacle, and (c) evaluating the data sets for obtaining quantitative three dimensional information.

Method and device for 3 dimensional imaging of suspended micro-objects providing high-resolution microscopy

A method is described for non-destructive measurement of vitality of biological cells, especially for determination of apoptosis, in which the at least one cell is exposed to high-frequency alternating, especially rotating, electric fields and/or impedance test fields, and at least one rotation measurement, one dielectrophoresis measurement and/or one impedance measurement is performed with the cell for at least one frequency range or individual frequencies, from which at least one measurement parameter is determined that is characteristic of the vitality state of the cell.

Method for measuring the vitality of cells

Described is a fluidic microsystem () including at least one channel () through which a particle suspension can flow; and first and second electrode devices () which are arranged on first and second channel walls () for generating electrical alternating-voltage fields in the channel (); wherein the first electrode device () for field shaping in the channel includes at least one first structure element (); and the second electrode device () includes an area-like electrode layer () with a closed second electrode surface which includes a second passivation layer (); wherein the effective electrode surface of the first structure element (), of which element () there is at least one, is smaller than the second electrode surface; and the second passivation layer () is a closed layer which completely covers the second electrode layer ().

Fluidic microsystem comprising field-forming passivation layers provided on microelectrodes

A method of discharging a fluid flow with suspended microparticles from a fluidic microsystem () is described, whereby the fluid flow converges with at least one output flow to form a discharge flow at the end of a discharge channel () of the microsystem, and the discharge flow is delivered through a conduction element (). A microsystem with a flow output device for implementation of this method is also described.

Method and device for withdrawing suspended microparticles from a fluidic microsystem

The invention relates to a method for generating a convective liquid motion in a fluidic microsystem. According to this method, a liquid in a microsystem is simultaneously exposed to an electrical field and a thermal gradient. The electrical field is generated by means of an electrode arrangement which is subjected to a time-variant voltage. In this way, a time variant electrical field is formed in the liquid volume. The thermal gradient is produced by means of at least one radiation absorber located in the compartment which is exposed to at least one external radiation field.

Method and device for generating microconvections

Described is a fluidic microsystem with at least one compartment for the receiving and/or the through-flow of a liquid and having an electrode arrangement with a multiplicity of electrodes, between which a coaction zone is established, whereby the compartment possesses at least one wall, through which electromagnetic radiation can be linked into the said coaction zone in accord with a predetermined incident direction, and on at least one electrode a cooling apparatus is provided, and on which at least one respective electrode at least one reflector layer is provided, and wherein the respective electrode is at least partially shielded in reference to the incident beam direction, and having at least one heat conducting layer by means of which the respective electrode stands in thermal communication with a wall of the compartment, and/or includes an active cooling element, which is placed in thermal contact with the respective electrode.

Microsystem for the dielectric and optical manipulation of particles

A microsystem adapted for dielectrophoretic manipulation of particles in a suspension liquid wherein the microsystem has a channel with channel walls and a longitudinal extension. An electrode arrangement is present which has at least one microelectrode on at least one of the channel walls. This acts to generate a field barrier which crosses the channel at least partly. The microelectrode has a band-shape or has a multitude of straight electrode sections connected to each other. The band-shape has a predetermined curvature or the straight electrode sections are arranged with predetermined different angles so that the field barrier has a predetermined curvature relative to the longitudinal extension of the channel.

Electrode arrangement for generating functional field barriers in microsystems

To measure or exert optically-induced forces on at least one particle in the focus of an optical cage, the following steps are taken:

Method and device for measuring, calibrating and using laser tweezers

For object detection, particularly in fluidic microsystems, optical imaging of at least one resting or moving object ( ) on a structured mask ( ) with at least one segment from a flat section ( ), in which the object ( ) is located at least partially or temporarily and which has a characteristic dimension smaller than the dimension of the object ( ) or its movement path, to a detector unit, detection of the quantity of light transmitted by the structured mask ( ), and generation of a detector signal which has a predetermined relationship with the quantity of light, and evaluation of the detector signal in regard to the presence of the object ( ), its position, its shape and/or the temporal change of the position are performed.

Process and device for the detection of microscopically small objects

Electrode arrangement ( ) in a microsystem adapted for dielectrophoretic manipulation of particles ( ) in a suspension fluid in a channel ( ), wherein at least one electrode ( ) is arranged on a lateral wall of the channel ( ), the electrode ( ) consisting of a plurality of electrode segments adapted to generate at least one field gradient for influencing the movement paths of the particles ( ) in the channel ( ). In one embodiment of the invention, the particles are moved in the microsystem by exposure to centrifugal and/or gravitational forces.

Growing community of inventors

Berlin, Germany

Thomas Schnelle