Waters Technologies Corporation

Thomas S McDonald

Joseph D Michienzi

Michael O Fogwill

Geoff C Gerhardt

Keith Fadgen

Martin Gilar

Sylvain Gilles Cormier

Joshua A Shreve

Abhijit Tarafder

Jeffrey Musacchio

Richard Wayne Andrews

Peyton C Beals

Rachel Mumma

Jordan Kornfeld

Joseph A Jarrell

Jason F Hill

Fabrice Gritti

Michael Eggertson

Mike Leal

Sample preparation and separation can be performed using a sample cartridge (). The cartridge includes a barrel () with a first and second end, a column segment () connected to the second end of the barrel, and a column () containing a sorbent material. The sorbent material includes particles that have antibodies attached to them to selectively retain analytes, proteins attached to them to retain certain classes of antibodies, or enzymes attached to them to perform specific modifications to certain classes of molecules. The column segment can be in thermal communication with a temperature control device in order to control the temperature of the column.

Micro solid phase extraction devices and methods

Methods and devices for the washing, extraction, and separation of a sample in a disposable chromatography cartridge () comprising a barrel () and a column (), and especially including reinforcement to the column permitting high-pressure separation.

The present disclosure relates to methodologies, systems, apparatus, and kits for controlling fluid flow within a chromatography system. A makeup pump is configured to pump a makeup fluid into the chromatography system downstream of the column. A first restrictor is located upstream of a detector and downstream of both the makeup pump and the column. Decreasing an output volume of the makeup pump can direct an output from the column through the first restrictor to the detector. Increasing an output volume of the makeup pump can direct the output from the column to a second restrictor located downstream of the makeup pump and the column and in parallel with the first restrictor and the detector.

System and method for controlling fluid flow within a chromatography system

The disclosure relates to a system for liquid sample introduction into a chromatography system. The system includes a syringe, a first valve in fluid communication with the syringe, a second valve in fluid communication with the sample, a vessel located between, and in fluid communication with, the first and second valves, a third valve in fluid communication with the first valve, the second valve and a chromatography column, and a pump in fluid communication with the third valve and a mobile phase. When the valves are in a first position the syringe draws the sample into the vessel. The mobile phase flows to the chromatography column. When the valves are in a second position, a portion of the mobile phase flows into the vessel, mixing with and pressurizing the sample. When the valves are in a third position, the mixed and pressurized sample flows to the chromatography column.

Mechanisms and methods for liquid sample introduction into a chromatography system

A method for injecting a diluted sample in a chromatography system includes merging a flow of a sample and a flow of a diluent to form a flow of a diluted sample. A dilution ratio of the diluted sample equals a sum of the volumetric flow rates of the sample and the diluent divided by the volumetric flow rate of the sample. The diluted sample is stored in a holding element before injection into a chromatographic system flow. Sample dilution occurs under low pressure relative to the chromatographic flow thereby allowing lower pressure sample and diluent syringes to be used. Other benefits include reduced compressibility and a reduction in leaks due to the lower pressure operation. The method avoids problems associated with manual techniques which can introduce errors due, for example, to loss of sample, sample precipitation and adsorption of sample to vials.

Method and apparatus for injecting a chromatographic sample

Described are a method and apparatus for diluting a chromatographic sample. The method includes repeating an alternating acquisition of sample fluidic plugs each having an incremental volume of sample and diluent fluidic plugs each having an incremental volume of diluent to obtain a stack of alternating sample and diluent fluidic plugs. The stack is inserted into a flow of a mobile phase in a chromatography system. Alternatively, the method includes repeating the steps of injecting an incremental volume of sample into a chromatographic system flow and providing an incremental volume of mobile phase into the chromatographic system flow. In either implementation, the dilution ratio of the sample equals the sum of the incremental volumes of the sample and the diluent or mobile phase divided by the sum of the incremental volumes of the sample.

The present disclosure relates to methodologies, systems and apparatus for controlling pressure in a CO-based chromatography system. A first COpump operates in constant flow mode and delivers COto a chromatography column, and liquid modifier is introduced to the chromatography column according to a gradient. A second COpump is disposed downstream of the column and operates in constant pressure mode to introduce COinto a flow stream at an output of the column. Liquid modifier is also introduced into the flow stream at the output of the column according to a reverse gradient compared to the gradient entering the chromatography column.

Carbon dioxide based chromatography systems including multiple carbon dioxide pumps

The present disclosure relates to methodologies, systems and apparatus for controlling pressure in a CO-based chromatography system. A first pressure control element is located downstream of a CO-based chromatography system and is disposed to control pressure within the column. A split restrictor is located downstream of the primary pressure control element and is disposed to divert a portion of the mobile phase flow to a detector. A second pressure control element is located downstream of the split restrictor and is disposed to control pressure at the restrictor. While the first pressure control element executes a pressure-controlled gradient separation, the second pressure control element maintains a constant pressure at the restrictor. During a composition-programmed gradient separation, the second control element maintains a constant pressure at the split restrictor while the first pressure control element maintains a constant average density across the column.

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Littleton, MA, United States of America

Thomas S McDonald