Qiagen Gmbh

Miltenyi Biotec B.v. Co., Kg

Preanalytix Gmbh

Qiagen Gaithresburg, Inc

Thomas Rothmann

Ralf Himmelreich

Christian Lenz

Roland Fabis

Andy Wende

Rainer Dahlke

Daniel Groelz

Gerd Grosshauser

Vera Hollaender

Irina A Nazarenko

Andreas Bosio

Christian Korfhage

Markus Sprenger-Haussels

Christoph Erbacher

Brian Lowe

Holger Engel

Vera Holländer

Arvind Virmani

Heinrich Spiecker

Gabriele Christoffel

Markus Jeziorski

Friederike Wilmer

Dominic O'Neil

Robert Pinard

Thomas Deutschmann

Shiuli Agarwal

Seiyu Hosono

Hans Robert Attig

Holly Basham

Hansueli Meyer

Emily Neil

Petra Benkel

Chris Nehme

Ryan Hindman

Thi My Chi Nguyen

Sabine Dorit Menzel

Cordula (Leurs) Homberg

Michael Eberhard

Josef Drexler

Eva Holzer

Thomas Laue

Sabine Hermes

Claudia Schmid

Andreas Schafer

Andreas Sch&auml;fer

Hans Attig

Sandra Jungpertz

Daniel GRÖLZ

Christian c/o Qiagen Gmbh Lenz

Cordula Leurs

Andreas Schäfer

Gerd Großhauser

Sabine Baumann

Microscopy imaging that allow for multiple mRNAs, proteins and metabolites to be spatially resolved at a subcellular level provides valuable molecular information which is a crucial factor for understanding tissue heterogeneity as for example within the tumor micro environment. The current invention describes a method (SUMI-Seq) which combines the use of Spatial Unique Molecular Identifier in situ sequencing and in vitro sequencing of rolonies derived from rolling circle amplification from padlock oligonucleotides targeting portion of RNA or cDNA transcript at a subcellular level with less limitation in the amount of transcripts and the length of the sequence that can be analyzed. Apart from padlocks oligonucleotides, the SUMI-Seq method can also be applied using circular oligonucleotides to spatially resolve proteins and metabolites to provide multiomics results.

Method and instrumentation for spatial multiomics using SUMI-technology

The present invention pertains inter alia to a method for analyzing a sample comprising biomolecules, which comprises the following steps: Furthermore, specifically adapted processing cartridges and systems are provided.

Sample processing method and sample processing cartridge

The invention relates to the preparation of a biological sample for performing verifications and examinations, wherein the aim of the invention is the creation of a method for preparing a biological sample having an improved PCR sensitivity compared to the reference standard having standard PCR without having to raise the cost thereof.

Preparation and amplification of nucleic acids by means of magnetic particles

Methods and materials for determining the presence of at least one nucleic acid in a sample are provided, said methods comprising (1) a purification step using sequence specific hybrid capture; (2) an amplification step; and (3) a detection step comprising contacting the target nucleic acid with a plurality of detectably labeled nucleic acid detection probes, wherein each (a) bears a different detectable label from the other detection probes, and/or (b) has a different melting temperature from probes bearing the same detectable label. Also disclosed are compositions and kits for use in such a method.

Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids

The present invention provides a method for fixing and/or stabilizing a sample, in which the sample is put into a permeable container, the permeable container having an opening, which is pressed into the sample, then a portion of the sample brought into the permeable container in this way is separated from the rest of the sample, and the container filled with the sample in this way is immersed in fixing and/or stabilizing agents and the sample is fixed and/or stabilized.

Method and device for fixing/stabilising a sample

The present invention relates to a method for amplifying a template nucleic acid, wherein the method comprises amplifying said template nucleic acid using the helicase dependent amplification (HDA) reaction in the presence of a nicking endonuclease, and wherein said template nucleic acid comprises a sequence recognized by said nicking endonuclease or a sequence recognized by said nicking endonuclease is introduced into the template nucleic acid during the HDA reaction. The invention further pertains to a kit for amplifying a nucleic acid, comprising a nicking endonuclease, a helicase and a DNA polymerase.

Helicase dependent isothermal amplification using nicking enzymes

The invention relates to a method and a device for the automatic processing of biological samples which can have a relatively large volume and which can be further processed by a microfluidic system. A container is provided with a filter and can be or is closed. A biological sample is introduced into the container. An inlet or outlet for liquids is mounted downstream of the filter. In order to carry out processing, liquids that are present in the container are not only sucked off through the filter and passed on via the inlet or outlet, but liquids required for processing are also pumped into the container through the filter. The container can be connected to a microfluidic system in a relatively easy manner since only one conduit is required for an automated processing.

Method and device for the automated processing of a sample

The present invention relates to a method for amplifying and detecting nucleic acid sequences in a reaction cartridge comprising, (i) providing a sample comprising at least one nucleic acid molecule, (ii) in a first reaction chamber of the cartridge providing reagents for an amplification reaction, (iii) mixing the sample with the amplification reagents, (iv) amplifying the at least one nucleic acid in the first reaction chamber of the cartridge, (v) transferring at least parts of the amplification reaction into a second and third reaction chamber of the cartridge each comprising a probe set and performing a melting point analysis in order to determine which of the probes has specifically bound a nucleic acid.

Detection of multiple nucleic acid sequences in a reaction cartridge

The present invention concerns a contamination barrierthat permits an efficient and reproducible processing of a high number of samples with the prevention of contamination of aqueous solutionsin open and/or automated systems, especially in the ppm range, in that it comprises at least one water immiscible hydrocarbon compound. In addition a method for the prevention of contamination during the processing of aqueous solutionsin open and/or automated systems is disclosed.

Contamination barrier

The present invention provides a method for fixing and/or stabilizing a sample, in which the sample is put into a permeable container with a maximum overall height of 10 mm, preferably of 5 mm, and the container filled with the sample is immersed in fixing and/or stabilizing agents and the sample is fixed and/or stabilized.

Method and device for fixing/stabilizing a sample

The invention relates to a method for breaking down biological material, especially for obtaining biomolecules by ultrasound, the biological material being arranged, in a container together with a liquid.

Method and device for breaking down biological material

The present invention relates to a device and a method for treating liquids with magnetic particles, wherein at least one further central element which ensures collection and homogenization of the particles is additionally provided.

Apparatus and method for the treatment of liquids with magnetic particles

Device for the separation of magnetic particles from a liquid, comprising a first vessel (), a second vessel (), a connecting surface () that runs from the interior of the first vessel () to the interior of the second vessel (), at least one magnet () for the provision of a magnetic field, and a guide element () by means of which the magnetic field can be guided along one side of the connecting surface ().

Device and method for the elimination of magnetic particles from a liquid

The present invention relates to the use of alkanes for the contamination-free purification or separation of biopolymers.

Use of alkanes for contamination-free purification or separation of biopolymers

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Langenfeld, Germany

Thomas Rothmann