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Basf Aktiengesellschaft

Sudzucker Aktiengesellschaft

University of Heidelberg

Thomas Rausch

Steffen Greiner

Hinrich Harling

Heiko Rosenkranz

Elke Duwenig

Silke Krausgrill

The invention relates to transgenic plants and plant cells comprising a reduced expression of invertase inhibitors. The modification of the expression of the invertase inhibitors is achieved by introducing a cDNA sequence in an antisense orientation with respect to a promoter. The expression of the antisense DNA sequence is under the regulation of either the CaMV35S promoter or a tissue specific promoter.

Transgenic plants and plant cells with reduced expression of invertase inhibitors

The present invention relates to a nucleic acid molecule encoding a vacuolar invertase and its use for modifying the plant saccharose metabolism, in particular in the vacuole of the cell of a plant storage organ. The present invention also relates to a fragment of the nucleic acid molecule, a vector containing the nucleic acid molecule or a fragment thereof, as well as a host cell, in particular a plant cell that contains the nucleic acid molecule or a fragment thereof. The present invention also comprises a transgenic plant, methods for producing a transgenic plant, as well as methods for modifying the saccharose metabolism of a plant, in particular of a plant part.

Nucleic acids coding for vacuolar invertases, vegetal cells and plants containing said nucleic acids and the use thereof

To express selection genes and resistance genes, it would be desirable to have available plant constitutive promoters which has a strong uniform constitutive activity in as many plant tissues or cell types as possible and which, moreover, show an even stronger activity, or are not repressed, under stress conditions. The invention provides DNA constructs which encompass a plant V-ATPase promoter which is operatively linked with a heterologous gene. The invention furthermore relates to the use of these constructs in the form of expression cassettes, recombinant vectors and in transgenic plants, plant cells or protoplasts. In particular the invention relates to the promoter of theV-ATPase subunit c isoform 2.

Plant gene expression under the control of constitutive plant V-ATPase promoters

Transgenic plants and plant cells comprising a reduced expression of invertase inhibitors

This invention relates to a nucleic acid that contains at least one nucleic acid sequence coding for a polypeptide, polypeptide being capable of reducing the enzymatic activity of an invertase; the polypeptide itself; and transgenic plants that contain this nucleic acid sequence. The invention further relates to methods of preparing such transgenic plants having reduced storage sucrose loss.

Growing community of inventors

Heidelberg, Germany

Thomas Rausch