Eastman-kodak Company

Johnson & Johnson Clinical Diagnostics, Inc.

Thomas R Kissel

Alan E Friedman

Richard L Columbus

Harold C Warren

Steven C Switalski

Lisa D Sprague

Gary L Snodgrass

Douglas R Jones

Brian A Snyder

Daniel S Daniel

Martin F Muszak

Paul B Contestable

Richard L Detwiler

Maurice A Kildal

Bradley P Boyer

Linda A Mauck

Brooke P Schlegel

Sarah Fingar Groulx

Martha Miller Kopcienski

Sarah A Fingar

An analytical element can be used to sensitively and rapidly detect a wide variety of specific binding ligands in either a competitive or sandwich assay format. The assays are carried out using a vanadium bromoperoxidase-labeled immunoreactant and a chemiluminescent signal-providing wash composition which comprises a 2,3-dihydro-1,4-phthalazinedione derivative; a halogen, pseudohalogen, halogen-providing source or pseudohalogen-providing source; and a peroxide or a peroxide-generating reagent composition.

Method for the determination of a specific binding ligand using a vanadium bromoperoxidase as a signal-generating enzyme

A simplified composition for generating a chemiluminescent signal in the presence of a peroxidase includes a substituted acetanilide as the sole light-producing substrate for the peroxidase. Assays for various analytes can be carried out with this composition at near neutral pH, exhibit low background and are highly sensitive.

Chemiluminescent reagent and assay using a substituted acetanilide for

There is disclosed a slide test element and method of assay that allows for detection of analytes in biological liquids without the use of any detection reagents. The method uses NIR spectroscopy and a test element comprising a support, and a substantially constant light-path defining layer comprising a diffusely-reflecting material that is a) sufficiently porous in all directions as to allow a liquid to spread uniformly in all directions, and b) homogeneously reflects at least 95% of NIR radiation.

Method for quantitative NIR spectroscopic analysis

Test element for quantitative NIR spectroscopic analysis

A buffered composition containing a nonionic or anionic surfactant, and a certain amount of a hydroxamic acid (or salt thereof) or an acyl hydrazine can be used as a wash composition in specific binding assays. Such assays include the use of a peroxidase labeled specific binding reactant to form a detectable peroxidase-labeled specific binding complex. The buffered composition and labeled reactant can be supplied in a diagnostic test kit. The hydroxamic acid or acyl hydrazine can be represented by the structure(I):

Wash composition containing signal stop reagent, test kit and method of

The signal generated in a specific binding assay wherein a peroxidase label is used to detect the resulting specific binding complex on a microporous filtration membrane can be modulated by contacting the signal forming reagents with a buffered solution of a hydroxamic acid or acyl hydrazine having the structure

Diagnostic test kit and specific binding assay using modulator of signal

A dry analytical element can be used to sensitively and rapidly detect a wide variety of specific binding ligands in either a competitive binding or sandwich assay format. The assays are carried out using a peroxidase-labeled immunoreactant. The peroxidase label is stabilized with a 4-hydroxy or 4-alkoxyarylacetamide which is located in one or more zones of the element. Not only is the label stabilized with the stabilizer, but the assay is more sensitive.

Analytical element and method for the determination of a specific

Aqueous compositions, test kits, test devices and methods can be used to detect hydrogen peroxide or peroxidase by generating a colorimetric or chemiluminescent signal in the presence of the analyte. Signal generation is enhanced by the presence of certain substituted 4-hydroxy- or 4-alkoxy-substituted phenyl or naphthyl electron transfer agents.

Use of 4'-hydroxy- and 4'-alkoxy-substituted electron transfer agents in

There are disclosed an ISE slide element and potentiometric measurement of assaying, using a slide element that is positionally insensitive about an axis of symmetry. This is achieved by accessing one of the electrodes in the center of the slide element, and the other in an annular groove centered on the element center, both for liquid contact and electrometer contact. Preferably, electrometer contact occurs at a side of the electrode opposite to the side contacted with liquid.

Round potentiometric slide elements and method of using the same

An aqueous composition useful for providing a chemiluminescent signal in analytical methods is stabilized by the presence of specific amounts of either a low molecular weight cationic surfactant or a cationic polymer. The composition also includes a 2,3-dihydro-1,4-phthalazinedione derivative, such as luminol, and 4'-hydroxyacetanilide. A chemluminescent signal is generated using the composition in the presence of peroxidase or a peroxidase-labeled specific binding species (such as an antibody or oligonucleotide).

Chemiluminescent composition containing cationic surfactants or polymers

A dressing device and method are described, for use with a needle that is injected into the skin. The dressing device comprises a cover sheet and a gel medium attached to the cover sheet, the gel medium being transparent and effective to reseal after being punctured by the needle. Most importantly, the gel medium includes a sterilizing agent. Injection of the needle into the skin occurs only after the needle penetrates the gel medium, so that the needle is disinfected or sterilized.

Sterilizing dressing device and method for skin puncture

There is disclosed a reference liquid for use with (a) a sample liquid to determine ionic activity of analytes, using differential analysis, and (b) two substantially identical dry-operative, ion-selective electrodes, the reference liquid comprising a solution mixture of (1) CsCl, and (2) an anion suitable for poising one of the electrodes for the analysis of the activity of CO.sub.2. A method of use is also described.

Reference liquid comprising CsCl

There are disclosed a process and the product produced by the process, of making an ion selective electrode for chloride. The process features, after forming a metal chloride layer, the step of washing the electrode in an organic acid, inorganic acid, or a solution of a salt of the acids having certain specified properties.

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Rochester, NY, United States of America

Thomas R Kissel