The United States of America As Represented by the Department of Health

The State University of New York

The United States of America

Thomas R Fuerst

Bernard Moss

Ira H Pastan

Tamio Mizukami

Edward A Berger

Vijay K Chaudhary

David Fitzgerald

David J FitzGerald

F William Studier

Vijay Kumar Chaudhary

Orna Elroy-Stein

Edward G Niles

A chimeric gene directing the synthesis of hybrid recombinant fusion protein in a suitable expression vector has been constructed. The fusion protein possesses the property of selective cytotoxicity against specific virus-infected cells. A CD4(178)-PE40 hybrid fusion protein has been made for selectively killing HIV-infected cells.

Cytotoxic agent against specific virus infection

A transient expression system is disclosed that utilizes bacteriophage RNA polymerase in the presence of a DNA-based cytoplasmic virus to facilitate expression of a foreign gene in the cytoplasm of a eukaryotic cell. A method of expressing a foreign gene in the cytoplasm of a eukaryotic cell is also disclosed which comprises incorporating into the cytoplasm a DNA-based cytoplasmic virus, a suitable carrier comprising a gene for an RNA polymerase which gene is foreign to the carrier and to the cells, and a suitable carrier comprising a functional, cistron including a foreign gene flanked by a promotor sequence which is recognized by the RNA polymerase.

Prokaryotic expression in eukaryotic cells

CD-4/cytotoxic gene fusions

An efficient, versatile and simple expression system which confers cap-independent translation to prokaryotic RNAs in eukaryotic cells has been described. The utility of recombinant vaccinia virus for this purpose has been illustrated.

Rapid, versatile and simple system for expressing genes in eukaryotic

Growing community of inventors

Gaithersburg, MD, United States of America

Thomas R Fuerst