Abbott Laboratories Corporation

Thomas P Leary

Suresh M Desai

George J Dawson

Isa K Mushahwar

Anthony Scott Muerhoff

John N Simons

Tami J Pilot-Matias

James Carl Erker

Sheri L Buijk

George G Schlauder

A Scott Muerhoff

Dinesh O Shah

Larry G Birkenmeyer

Robin A Gutierrez

James L Stewart

Michelle Chalmers

Lily Jiang

Mark A Hayden

Andrew G Fischer

Jeffrey B Huff

Qiaoqiao Ruan

Sergey Y Tetin

Ronald L Marshall

Mark R Pope

Shelley R Holets-McCormack

Sophie Laurenson

Robert J Carrick

Pathik H Soni

Lyle Yarnell

Cynthia Jou

John F Uher

Lei Qiao

M Felicia Bogdan

Ramani S Wonderling

Dustin House

Peter J Karabatsos

Edna Prieto-Ballengee

You Pan

Richard Haack

Teresa K Surowy

Robin A Guetierrez

Andrew S Schapals

Scott Muerhoff

M Shawn Murphy

Stefan Hershberger

Xingli Jiang

George A Dawson

Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided.

Devices and methods for sample analysis

A polypeptide comprising the contiguous amino acids 1-198 of SEQ ID NO: 2; a polypeptide, which comprises a contiguous amino acid sequence that is at least about 95% identical to the contiguous amino acids 1-198 of SEQ ID NO: 2, an epitope that is immunoreactive with an antibody that specifically binds to the core protein of hepatitis C virus (HCV), and an epitope that is immunoreactive with an antibody that specifically binds to the NS4 region of HCV; a nucleic acid encoding such a polypeptide; a host cell comprising such a nucleic acid; an immunodiagnostic reagent comprising such a polypeptide; a kit comprising such an immunodiagnostic reagent; and a method of determining the presence, amount, or concentration of anti-HCV antibodies in a test sample.

Materials and methods for assay of anti-hepatitis C virus (HCV) antibodies

The present invention relates to a new method of producing functionally active recombinant porcine Intrinsic Factor as well as the protein (i.e., porcine Intrinsic Factor) produced thereby. In particular, the vector comprising the DNA encoding the protein is introduced into a Chinese Hamster Ovary (CHO) host cell under conditions sufficient for optimal expression of functional protein. The expressed protein may then be subjected to three methods ultimately resulting in a protein having at least 97% purity.

Cloning, expression and purification of recombinant porcine intrinsic factor for use in diagnostic assay

The subject invention relates to methods for the simultaneous detection of Hepatitis C Virus (HCV) antigens as well as antibodies produced in response to HCV antigens. Furthermore, the subject invention allows one to detect antigens in the early, acute stage of infection, even prior to the development of antibodies, thereby allowing for early detection of infected blood and blood products, thus improving the safety of the blood supply.

Methods for the simultaneous detection of HCV antigens and HCV antibodies

Hepatitis GB Virus (HGBV) nucleic acid and amino acid sequences useful for a variety of diagnostic and therapeutic applications, kits for using the HGBV nucleic acid or amino acid sequences, HGBV immunogenic particles, and antibodies which specifically bind to HGBV. Also provided are methods for producing antibodies, polyclonal or monoclonal, from the HGBV nucleic acid or amino acid sequences.

Non-a, non-b, non-c, non-c, non-d, non-e hepatitis reagents and methods for their use

Non-A, non-B, non-C, non-D, non-E hepatitis reagents and methods for their use

The subject invention relates to improvements in nucleic acid isolation, and more particularly, relates to modifications to the subtractive hybridization method and to reagents such as oligonucleotides that are useful when performing the method.

Method of performing subtractive hybridization using RDA

The present invention relates to nucleic oligomer primers or probes useful for detection of TTV in test samples. Also provided are assays which utilize these primers and probes, as well as test kits which contain these oligomer primers and/or probes. In addition, the present invention encompasses the use of TTV nucleotide sequences as nucleic acid vectors and as markers for determining transmission between individuals as well as the route thereof. Additionally, the present invention encompasses a method of detecting TTV infection prior to xenotransplatation of a tissue or organ.

Methods of utilizing the TT virus

Recombinantly produced hepatitis GB Virus (HGBV) amino acid sequences useful for a variety of diagnostic and therapeutic applications, kits for using the HGBV amino acid sequences and antibodies which specifically bind to HGBV. Also provided are methods for producing antibodies, polyclonal or monoclonal, from the HGBV recombinantly produced amino acid sequences.

Hepatitis GB virus recombinant proteins and uses thereof

Non-A, non-B, non-C, non-D, non-E hepatitis reagents and methods for

Hepatitus GB Virus (HGBV) nucleic acid and amino acid sequences useful for a variety of diagnostic and theraeutic applications, kits for using the HGBV nucleic acid or amino acid sequences, HGBV immunogenic particles, and antibodies which specifically bind to HGBV. Also provided are methods for producing antibodies, polyclonal or monoclonal, from the HGBV nucleic acid or amino acid sequences.

Non-A, non-B, non-C, non-D, non-E Hepatitis reagents and methods for

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Kenosha, WI, United States of America

Thomas P Leary