University of Pittsburgh

The United States of America, As Represented by the Secretary, Department of Health and Human Services

University System of Maryland

The United States Government As Represented by the Department of Veterans Affairs

Thomas P Conrads

Soldano Ferrone

Xinhui Wang

Elvira Favoino

Brian L Hood

Timothy D Veenstra

Christopher J Michejda

Susan K Keay

Zoltan Szekely

Haleem J Issaq

George Janini

Kenneth L Wilkens

Gillian Tocci

Brian Lewis Hood

Isolated monoclonal antibodies are disclosed herein that specifically bind endoplasmin. In some embodiments these antibodies are fully human. Recombinant nucleic acids encoding these antibodies, expression vectors including these nucleic acids, and host cells transformed with these expression vectors are also disclosed herein. In several embodiments the disclosed antibodies are of use for detecting and/or treating tumors that express endoplasmin, such as melanoma, breast cancer, head and neck squamous cell carcinoma, renal cancer, lung cancer, glioma, bladder cancer, ovarian cancer or pancreatic cancer. In one example, the tumor is a melanoma.

Antibodies to endoplasmin and their use

A novel antiproliferative factor comprising a glycopeptide is disclosed. In specific embodiments, the novel antiproliferative factor is associated with the bladder. Compositions, diagnostic kits and reagents, and methods of using the compounds for identifying and/or treating interstitial cystitis and cancer are disclosed.

Antiproliferative factor and methods of use

Contiguous capillaries useful for separating and electrospraying a fluid comprising analyte and electrolyte are provided. The contiguous capillaries have spray tips at one end of the capillaries and electrically conductive portions in proximity to the spray tips. Methods for making the contiguous capillaries and their use as electrospray sources are also disclosed. Apparatus and methods for conveying analyte ions from the capillaries into analytical instruments, such as a mass spectrometer, are also disclosed. The disclosed contiguous capillaries may be used to carryout electrophoresis separation and electrospray ionization of analytes. Methods for obtaining the mass spectra of macromolecular analytes at concentrations lower than previously possibly are provided using the apparatus and procedures described herein.

Contiguous capillary electrospray sources and analytical devices

Growing community of inventors

Reston, VA, United States of America

Thomas P Conrads