Carl Zeiss Microscopy Gmbh

Cad Zeiss Microscopy Gmbh

Thomas Ohrt

Daniel Haase

Markus Sticker

Manuel Amthor

Michael Goegler

Lars Stoppe

Thomas Kalkbrenner

Thorsten Kues

Ralf Wolleschensky

Ingo Fahlbusch

Johannes Knoblich

Christian Dietrich

Joerg Siebenmorgen

Michael Gögler

Sebastian Backs

System for state monitoring of a microscope the system having at least one measuring sensor in each case for capturing at least one time-variable chemical and/or physical quantity, a camera for recording an image in a field of view and a processing unit. The at least one measuring sensor has a display area and displays thereon a measured value for the captured time-variable chemical and/or physical quantity. The camera is arranged so that the display areas of at least one measuring sensor are located in the field of view and the processing unit is configured to evaluate the image and to extract the display areas contained in the image therefrom. Also, a method for state monitoring of a microscope is disclosed, wherein at least one measuring sensor with a display area is provided in order to capture in each case at least one time-variable chemical and/or physical quantity, and an image is recorded. The image is recorded so that it contains the display areas of at least one measuring sensor. The display areas are identified in the image, the image is evaluated and the measured values contained in the image are extracted.

System and method for monitoring of states of components of a microscope

A method for preparing a microscope for imaging a sample. The method includes the following steps: providing the microscope for imaging the sample, wherein the microscope comprises an objective having a motor-adjustable objective correction ring for correcting imaging aberrations; acquiring sample information comprising at least one of the following indications: thickness of a cover glass, material of the cover glass, sample temperature, sample type, sample location on a sample carrier, embedding medium of the sample, or immersion medium; imaging the sample using the microscope for generating at least one raw image of the sample with a first setting of the objective correction ring; and inputting the at least one raw image and the sample information into a machine algorithm and determining a second setting of the objective correction ring, which reduces the imaging aberrations vis à vis the first setting, by means of the algorithm on the basis of the raw image and the sample information.

Method of preparing a microscope for imaging a sample

A microscopy system comprises a microscope with an overview camera for capturing at least one overview image of a sample carrier designed to receive at least one sample fluid; and a computing device configured to determine at least one sample image area of the at least one sample fluid within the at least one overview image. The computing device comprises an evaluation program into which a determined sample image area is entered and which is configured to determine a fluid state of the associated sample fluid based on the sample image area.

Microscopy system and method for analyzing an overview image

For the color correction of microscope images, an object that corresponds to a predetermined object type of a known color is localized in a microscope image using an image processing program. Based on an image region of the localized object, a color correction is determined with which a color of the localized object in the image region is brought into accordance with the known color. The color correction is applied to at least one section of the microscope image or of another microscope image or is used in a capture of a further microscope image.

Microscopy system and method for the color correction of microscope images

Provision is made for a method of monitoring an immersion fluid in a microscope having a lens which images a sample located on a sample carrier. In a step a), a camera is positioned which has an image field which is oriented in such a way that it captures the sample carrier and a space between the sample carrier and the lens and adjoining the sample carrier towards the lens, which space is used to receive the immersion fluid. In a step b), the immersion fluid is applied into the space between the sample carrier and the lens. In step c), an image with the immersion fluid being in the space between the sample carrier and the lens is recorded, and in a step d), the position, the area and/or the contour of the immersion fluid on the sample carrier from the image recorded in step d) are/is determined.

Method for monitoring an immersion fluid in a microscope

A method for generating an overview image of a sample which is arranged in an observation volume of a microscope by means of a sample carrier is proposed, wherein the sample carrier is illuminated by a first illumination, wherein a preliminary overview image is generated using the first illumination and an overview camera of the microscope, wherein an overview image illumination is chosen on the basis of the preliminary overview image, wherein the sample carrier is illuminated by the overview illumination, and wherein the overview image is generated using the overview image illumination and the overview camera.

Method and microscope for generating an overview image of a sample

A method for processing microscope images comprises: acquiring a microscope image captured by a microscope; identifying at least one image section with sensitive information within the microscope image by means of an image processing program which uses provided reference information regarding sensitive information; rendering unrecognizable the at least one identified image section with sensitive information in order to generate an anonymized image; and outputting the anonymized image.

Microscopy system, method and computer program for processing microscope images

At least one image is obtained which images an external view of at least one interchangeable component (-) of an optical system (). Contextual information for the at least one interchangeable component (-) is determined on the basis of the at least one image.

Determination of contextual information for interchangeable components of an optical system

A method for regulating a light source of a microscope that illuminates an object, said method including specifying an intended value of an energy parameter of illumination radiation on the object; producing illumination radiation; providing an objective for focusing illumination radiation onto the object; ascertaining a transmission property of the objective for the illumination radiation; output coupling a component of the illumination radiation upstream of the objective as measurement radiation and measuring an actual value of the energy parameter of the measurement radiation; providing a relationship between energy parameters of the measurement radiation and energy parameters of the illumination radiation on the object, and setting the light source in such a way that the actual value of the energy parameter measured for the measurement radiation corresponds to the intended value of the energy parameter according to the relationship.

Method for controlling or regulating a microscope illumination

A method for the microscopy scanning of a specimen. An immersion medium is used between a slide and a microscope objective, said immersion medium wetting a surface of the slide, and the microscope objective being relatively displaced over the surface of the slide for imaging. The surface is provided with a coating which repels the immersion medium.

Scanning immersion microscopy

A microscope objective for imaging a specimen using a microscope, the microscope objective having a front lens enclosed by a surround and being designed for microscopy with an immersion liquid. In the microscope objective, the front lens and/or the surround thereof is provided with a coating which can be switched between a state which repels the immersion liquid and a state which does not repel the immersion liquid.

Immersion microscopy

An optical apparatus having an illumination module with a carrier, which has at least one light-transmissive region, for example. The illumination module has a plurality of light sources, which are arranged on the carrier.

Illumination module for angle-selective illumination

A microscope objective for imaging a specimen using a microscope, the microscope objective being designed as an air objective for microscopy without an immersion medium or as an oil immersion objective for microscopy with an oil-based immersion medium or as a water immersion objective for microscopy with a water-based immersion medium. The front lens of the microscope objective is provided with a coating which repels an immersion medium and is lipophobic and hydrophobic if the objective is an air objective, only lipophobic if the objective is a water immersion objective, and only hydrophobic if the objective is an oil immersion objective.

Microscope objective and microscope having such an objective

A method for optically examining a plurality of microscopic samples. The samples are channeled one after the other by means of a flow into at least one flow channel in which the samples advance along a flow direction. The samples are illuminated, and light emitted from the samples is detected and analyzed. A device for carrying out the method in which samples are illuminated in that at least one light sheet with a light sheet plane is directed onto the at least one flow channel, wherein the light sheet is oriented so as to intersect the at least one flow channel in an intersection region, and the normal of the light sheet plane forms an angle differing from null together with the flow direction in the intersection region. The light emitted from the sample is registered by an imaging optical detection unit, and the focal plane of said optical detection unit lies in the intersection region.

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Golmsdorf, Germany

Thomas Ohrt