Wyeth

Other

American Home Products Corporation

Berlex Laboratories, Inc.

Thomas Michael Argentieri

Jeffrey Howard Sheldon

Mark Robert Bowlby

Qiang Wang

Thomas J Fisher

Disclosed herein are nucleic acid and polypeptide sequences of a novel rat voltage-gated potassium channel, KCNQ5. Also disclosed herein are methods related to the use of the aforementioned potassium channel.

Rat KCNQ5 voltage-gated potassium channel

The present invention involves an assay system and method of selecting compounds useful in the treatment of bladder instability and related bladder conditions through the activation of KCNQ potassium channels in the bladder smooth muscle.

Methods of selecting compounds for modulation of bladder function

This invention provides methods for the treatment or inhibition of hyperactive gastrointestinal motility in a mammal utilizing compounds having the formula:where R, and Rto Rare described herein.

Methods for treating hyperactive gastrointestinal motility

This invention provides methods and pharmaceutical compositions for maintaining bladder control or treating urinary incontinence in a mammal utilizing agonists of KCNQ potassium channels, including KCNQ2, KCNQ3, KCNQ4 and KCNQ5 potassium channels, alone or in combination. Compounds useful in these methods include the 1,2,4-triamino-benzene derivatives described in U.S. Pat. No. 5,384,330 (Dieter et al.) and the substituted 3-phenyl oxindole compounds described in U.S. Pat. No. 5,565,483 (Hewawasam et al.). Among the preferred compounds of this invention is N-&lsqb;2-amino-4-(4-fluorobenzylamino)-phenyl&rsqb;carbamic acid ethyl ester, also referred to as retigabine.

Methods for modulating bladder function

A circular tissue recording chamber has a cavity therein for retaining tissue or cell samples immersed in a liquid bath. The temperature of the bath is maintained at a selected level by a transparent heater positioned beneath the bath, wherein the heater is comprised of a grid of fine wires supported on a transparent mylar substrate. Liquid is introduced into the bath through a serpentine passageway also in proximity with the heater, whereby the temperature of the liquid is raised to that of the bath as it traverses the passageway. The recording chamber has a thickness of approximately 1 cm so as to be readily usable with a high magnification microscope.

Heated recording chamber

A modular tissue superfusion chamber includes a receptacle support having a recess therein for receiving one of a plurality of modular bath containers which hold tissue samples being tested for electrophysiological responses to compounds. The receptacle support has a Peltier heater therein and is mounted on a magnetic base with a plurality of pillars.

Growing community of inventors

Yardley, PA, United States of America

Thomas Michael Argentieri