Dow Agrosciences LLC

Agrigenetics, Inc.

Doe Agrosciences LLC

Corteva Agriscience LLC

Thomas Meade

Kenneth Edwin Narva

Aaron Todd Woosley

Joel Jay Sheets

Stephanie L Burton

Timothy Denver Hey

Nicholas P Storer

Donald J Merlo

Todd P Glancy

Sek Yee Tan

Justin Michael Lira

Sarah E Worden

Diaa Alabed

Patricia C Apel-Birkhold

Karen C McLaughlin

Jim Xinpei Huang

Sean Michael Russell

Jon Christopher Mitchell

Kristin J Fencil

Joel J Sheets

Robin Leola Thompson

Ze-Sheng Li

Aaron T Woosley

Kenneth Narva

Yuanming Zhu

Thomas Clarence Sparks

Michael R Loso

Richard Brewer Rogers

Ignacio Mario Larrinua

Baolong Zhu

James Michael Gifford

Scott Bevan

Holly Jean Butler

Nailah Orr

Doug A Smith

Jonathan M Babcock

Scott B Bintrim

Qihua C Cai

Weiting W Ni

Ze Sheng Li

Amanda D Schleper

Haley Jo Moon

Marc Farrow

Diane M Retallack

Monica Britt Olson

Huarong Li

James Edward King

Krishna M Madduri

Gaofeng Lin

Ryan M Lee

Janna Mai Armstrong

Audrey Jane Etter

Jianquan Li

Tristan E Coram

Jon C Michell

James E King

Timothy D Hey

Doug Smith

JimX Huang

Stephenie L Burton

Karen C Mclaughlin

Jim X Huang

Stephanie Burton

The subject invention relates in part to the surprising discovery that CryDa is active against corn earworm (CEW),(Boddie). Methods for using CryDa in transgenic plants to prevent serious crop damage is described. Leaf and silk bioassays using transgenic maize expressing full length, core toxin region or chimeric CryDa demonstrated good insect protection against CEW larvae damage.

Cry1D for controlling corn earworm

The subject invention relates in part to the surprising discovery that Cry1Da is active against corn earworm (CEW),(Boddie). Methods for using Cry1Da in transgenic plants to prevent serious crop damage is described. Leaf and silk bioassays using transgenic maize expressing full length, core toxin region or chimeric Cry1Da demonstrated good insect protection against CEW larvae damage.

The subject invention concernsmodified Cry1Ca insecticidal toxins and the polynucleotide sequences which encode these toxins. Uses in transgenic plants are described as are methods for protecting crops from insect pest damage.

Modified Cry1Ca toxins useful for control of insect pests

The subject invention includes methods and plants for controlling European corn borer, said plants comprising a Cry1Ab insecticidal protein and a DIG-3 insecticidal protein to delay or prevent development of resistance by the insect.

Use of DIG3 insecticidal crystal protein in combination with cry1Ab for management of resistance in european cornborer

CRY1D for controlling corn earworm

The subject invention includes methods and plants for controlling fall armyworm lepidopteran insects, said plants comprising a Cry1Da insecticidal protein and a Cry1Ca insecticidal protein, and various combinations of other proteins comprising this pair of proteins, to delay or prevent development of resistance by the insects.

Use of Cry1Da in combination with Cry1Ca for management of resistant insects

The subject invention relates in part to Cry3Aa in combination with Cry6Aa. The subject invention relates in part to the surprising discovery that combinations of Cry3Aa and Cry6Aa are useful for preventing development of resistance (to either insecticidal protein system alone) by a corn rootworm (spp.) population. Included within the subject invention are plants producing these insecticidal Cry proteins, which are useful to mitigate concern that a corn rootworm population could develop that would be resistant to either of these insecticidal protein systems alone. The subject invention also relates in part to combinations of Cry3Aa and Cry6Aa proteins 'triple-stacked' or 'multi-stacked' with another insecticidal protein(s) such as a Cry6Aa protein or binary Cry34/35 proteins. Thus, such embodiments target rootworms with three modes of action. Transgenic plants, including corn, comprising a cry6Aa gene and a cry3Aa gene are included within the scope of the subject invention.

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The subject invention includes methods and plants for controlling European corn borer and/or fall armyworm insects, said plants comprising a Cry1Ab insecticidal protein and a Cry1Be insecticidal protein, and various combinations of other proteins comprising this pair of proteins, to delay or prevent development of resistance by the insects.

Use of Cry1Ab in combination with Cry1Be for management of resistant insects

strains that harbor transformation-enhancing genes on a plasmid capable of replication independently of thechromosome, the Ti plasmid, and plant transformation binary vectors, and uses for thesestrains are provided. Additionally,strains that are deficient in DNA recombination functions that result in instability or rearrangement of plant transformation binary vectors, and that harbor transformation-enhancing genes on a plasmid capable of replication independently of thechromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these strains, are also provided. Further included arestrains that harbor transformation-enhancing genes integrated into thechromosome at a locus that does not interfere with or otherwise compromise the normal growth and plant transformation ability of thecells, and uses for thesestrains. Plants made using thesestrains are also described.

The subject invention includes methods and plants for controlling lepidopteran insects. The plants comprise a Cry1Fa insecticidal protein and a Cry1Ca insecticidal protein, in combination, to delay or prevent development of resistance by the insect(s).

Combined use of CRY1Ca and CRY1Fa proteins for insect resistance management

The subject invention relates in part to stacking Cry1Be toxins along with Cry1Fa toxins to prevent insects from developing resistance towards either toxin by itself. As discussed in more detail herein, the subject pair of proteins is a particularly advantageous combination, as no other pair of proteins is known to provide high levels of control and non-cross-resistant activity against both(FAW) and(ECB) insects. This dual, non-cross-resistant activity is also advantageous because it can reduce the number of proteins/genes needed to target these insects with multiple, non-cross-resistant proteins. This can reduce or eliminate the need for refuge acreage. Accordingly, the subject invention also relates generally to using four genes to provide three proteins for non-cross-resistant control of a first insect, and three proteins for non-cross-resistant control of a second insect. In preferred embodiments, the targeted insects are FAW and ECB.

Insect resistance management with combinations of Cry1Be and Cry1F proteins

The subject invention includes methods and plants for controlling fall army worm insects, said plants comprising a Cry1Da insecticidal protein and a Cry1Be insecticidal protein, and various combinations of other proteins comprising this pair of proteins, to delay or prevent development of resistance by the insects.

Use of Cry1Da in combination with Cry1Be for management of resistant insects

The present invention includes modified, insecticidal B.t. Cry1Ca proteins, including the proteins designated herein as DIG-109 and DIG-152, as well as variants of DIG-109 and DIG-152, nucleic acids encoding these proteins, methods of controlling pests using the proteins, methods of producing the proteins in transgenic host cells, and transgenic plants that produce the proteins. The DIG-109 and DIG-152 proteins comprise chimeric peptides composed of a core toxin segment of B.t. Cry1Ca and a Cry1Ab protoxin segment. Insecticidally active variants of the DIG-109 and DIG-152 proteins are also described.

Modified Cry1Ca insecticial Cry proteins

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Zionsville, IN, United States of America

Thomas Meade