Commonwealth Scientific and Industrial Research Organisation

Thomas Mark Boyce

John Graham Oakeshott

Robyn Joyce Russell

Peter Malcolm Campbell

Richard David Newcomb

Kerrie-Ann Smyth

Geoffrey Charles De Quetteville Robin

Jeremy Colin Brownlie

Charles Claudianos

Kerrie-Anne Smyth

Geoffrey Charles Robin

Anthony Gerard Parker

The present invention provides an isolated DNA molecule encoding Malathion Carboxylesterase capable of hydrolysing at least one organophosphate selected from the group consisting of carboxylester organophosphates and dimethyloxon organophosphates. The DNA molecule comprises a nucleotide sequence having at least 60%, preferably at least 80% and more preferably at least 95% homology with Lc?E 7, in which the protein encoded by the DNA molecule differs from E 3 at least in the substitution of Trip at position 251 with an amino acid selected from the group consisting of Leu, Ser, Ala, Ile, Val, Thr, Cys, Met and Gly. The preferred substituents are Leu and Ser.

Malathion carboxylesterase

The present invention provides an isolated DNA molecule encoding Malathion Carboxylesterase capable of hydrolysing at least one organophosphate selected from the group consisting of carboxylester organophosphates and dimethyloxon organophosphates. The DNA molecule comprises a nucleotide sequence having at least 60%, preferably at least 80% and more preferably at least 95% homology with Lc&agr;E,, in which the protein encoded by the DNA molecule differs from E,at least in the substitution of Trip at position,with an amino acid selected from the group consisting of Leu, Ser, Ala, Ile, Val, Thr, Cys, Met and Gly. The preferred substituents are Leu and Ser.

Enzyme based bioremediation

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Syracuse, NY, United States of America

Thomas Mark Boyce