Becton Dickinson and Company Limited

Thomas L Fort

Ray A McMillian

Matthew P Collis

Qimin You

Tobin J Hellyer

Thomas M Gentle

Donald W Copertino

Oscar J Llorin

Anne B Brown

Karen Eckert

Bradley Scott Thomas

Timothy Roy Hansen

Dolores M Berger

William A Nussbaumer

Ray A Mcmillian

Jr Thomas M Gentle

Matthew P Collins

A system and method for preparing and testing of targeted nucleic acids is presented. The system integrates a pipetter, extractor, assay reader, and other components, including a selectively compliant articulated robot arm (SCARA). This synergistic integration of previously separate diagnostic tools creates a system and method whereby a minimum of human intervention is required. The resulting system provides a substantially more accurate and precise method of isolating, amplifying and detecting targeted nucleic acids for diagnosing diseases.

Automated system for isolating, amplifying and detecting a target nucleic acid sequence

The present invention relates to a method of ensuring the effectiveness of the extraction workup from a biological sample of nucleic acid. The inventive method is able to distinguish between possible defects in the extraction of nucleic acid from a sample and possible defects in a subsequent amplification step. The present invention also relates to a packaged array for extracting nucleic acid from a biological sample.

Use of an extraction control in a method of extracting nucleic acids

The present invention relates to methods for pretreating biological samples for extraction of nucleic acid therefrom. The present invention employs a combination of at least one protein denaturant with one or more of the following elements to form a reaction mixture for extraction of nucleic acid: (1) at least one aprotic solvent, (2) stepwise heating, and (3) sample dilution.

Pretreatment method for extraction of nucleic acid from biological samples and kits therefor

The present invention relates to methods for pretreating biological samples for extraction of nucleic acid therefrom The present invention employs a combination of at least one protein denaturant with one or more of the following elements to form a reaction mixture for extraction of nucleic acid: (1) at least one aprotic solvent, (2) stepwise heating, and (3) sample dilution.

Primers and probes derived from the HBV DNA polymerase gene which facilitate detection and/or quantification of all presently known genotypes of HBV. Disclosed sequences may be used in a variety of primer and probe constructs for detection of HBV nucleic acids.

Sequences and methods for detection of hepatitis B virus

The present invention relates to methods in which ion exchange resins are used to reduce the amount of substances which interfere with nucleic acid hybridization in samples. The methods also stabilize the samples. Kits containing the ion exchange resins render the methods convenient to use.

Sample processing method using ion exchange resin

Amplification primers and methods for specific amplification and detection of a,target are disclosed. The primer-target binding sequences are useful for amplification and detection of,target in a variety of amplification and detection reactions.

Amplification and detection of Yersinia enterocolitica

Amplification primers and methods for specific amplification and detection of a Campylobacter jejuni and C. coli target are disclosed. The primer-target binding sequences are useful for amplification and detection of C jejuni and C. coli target in a variety of amplification and detection reactions.

Amplification and detection of Campylobacter jejuni and Campylobacter

Amplification primers and methods for specific amplification and detection of a Shiga-like toxin I (SLT-I) target are disclosed. The primer-target binding sequences are useful for amplification and detection of SLT-I target in a variety of amplification and detection reactions.

Amplification and detection of shiga-like toxin I producing organisms

Amplification primers and methods for specific amplification and detection of a Shiga-like toxin II (SLT-II) target are disclosed. The primer-target binding sequences are useful for amplification and detection of SLT-II target in a variety of amplification and detection reactions.

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Hanover, PA, United States of America

Thomas L Fort