Basf Se Corporation

Thomas Kaeding

Wolfgang Siegel

Hartwig Schröder

Christopher Koradin

Hartwig Schroeder

Hartwig Schroder

Manfred Ehresmann

Melanie Bonnekessel

Andreas Pletsch

Stephan Freyer

Petra Deckert

Vaidotas Navickas

Franz Weber

Wolfgang Reichert

Kerstin Hage

Burkhard Ernst

Peter Oedman

Stefanie Demming

Carsten Schwalb

Katrin Treier-Marxen

Andreas Schaedler

Ralf Hoock

Sebastian Briechle

Karin Schein-Albrecht

Volker Wengert

Gunter Steigelmann

Peter Odman

Peter ÖDman

Hartwig SCHRÖDER

The present invention is directed to a method of purifying a protein of interest from the particulate matter of a fermentation broth comprising the step of purifying the protein of interest from the particulate matter of the fermentation broth, wherein the step of purifying the protein of interest from the particulate matter of the fermentation broth comprises one or more washing steps, which comprises contacting the particulate matter with a washing solution comprising one or more conditions that favor the solubilization of the protein of interest and/or the desorption of the protein of interest from the particulate matter.

Method of purifying a protein from fermentation solids under desorbing conditions

The invention pertains to processes to produce dry biomass of pyripyropene producer organisms, processes to obtain pyripyropenes from such dry biomass, as well as to processes to produce compounds of Formula III and/or Formula IV and/or Formula V from the pyripyropenes obtained from the dry biomass. The invention does further pertain to the dry biomass itself, as well as processes using said dry biomass to obtain pyripyropenes for the production of compounds of Formula III and/or Formula IV and/or Formula V, including processes using said dry biomass to obtain pyripyropenes or compounds of Formula III and/or Formula IV and/or Formula V in order to produce pest control compositions, in particular insecticides, comprising such compounds.

Production of pyripyropenes from dry biomass

The present invention relates to a method for preparing the pyripyropene compound of the formula (I) which method comprises the following steps: i) subjecting a pyripyropene compound of formula Pyripyropene A to an alkaline hydrolysis to yield a 1,7,11 -trideacetylpyripyropene A, ii) reacting 1,7,11-trideacetylpyripyropene A obtained in step i) with cyclopropane carbonyl chloride to yield a raw product containing the pyripyropene compound of formula (I); iii) subjecting the raw product of step ii) to crystallization to yield a crystalline pyripyropene compound of formula (I) and a mother liquor; and iv) recycling the mother liquor or a pyripyropene compound containing fraction thereof to the alkaline hydrolysis of step i). 

Method for preparing a pyripyropene compound

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Mannheim, Germany

Thomas Kaeding