Ge Healthcare Bio-sciences Corp.

Global Life Sciences Solutions USA LLC

Roche Vitamins Inc.

General Electric Company

Sartorius Stedim Biotech Gmbh

Dsm Nutritional Products Inc.

Thomas John Erdenberger

Richard Lee Damren

Colin R Tuohey

Parrish M Galliher

Michael Edward Fisher

Jonathan A Kenney

Joseph D Crowell

Patrick M Guertin

Kenneth P Clapp

Janice G Pero

Geoffrey L Hodge

Theron Hermann

Patrik Berndt Akerstrom

John B Perkins

Alan Sloma

Randolph T Hatch

David Lawrence Brazell

Wei Jin

Roberto Miguel Cabral

Sampath Telikcherla Kandala

John Swibes

Peter A Mitchell

Colin Touhey

Radislav Alexandrovich Potyrailo

Victoria Eugenia Cotero

Patric Fricking

Jon Albert Dieringer

Timothy A Wortley

Ted Deloggio

Parish M Galliher

Jiyoung Lee

Patrick Guertin

Timothy Wortley

Sampath Telikherla Kandala

Peter Mitchell

Disclosed herein is a single use continuous recovery, flow-through harvest vessel and corresponding method for harvesting culture medium and simultaneously either leaving the microcarrier beads behind in the vessel or flowing microcarrier beads and medium back into a bioreactor.

Continuous recovery harvest bag

A bioprocess mixer (), which comprises: &#x2014;a support vessel () with at least one side wall () and a bottom wall (), where the walls define a support vessel inner volume (), and at least a first () and a second () magnetic impeller drive unit; and &#x2014;a flexible bag () adapted to fit inside the support vessel inner volume, where the bag has at least one bag side wall (), a bag bottom wall () and a bag top wall () defining a bag inner volume (), and at least a first () and a second () magnetic impeller rotatably attached to a bag wall in the bag inner volume.

Bioprocess mixer

A probe assembly for inserting a probe into a flexible or semi-rigid object is provided. The probe assembly includes an aseptic connector for coupling to the object, a probe sheath that extends longitudinally from the aseptic connector, and an actuator for deploying the probe within the object. The probe sheath has a plurality of concentric sheath elements and an inner longitudinal volume that is configured to permit longitudinal movement of the probe within the probe sheath. The actuator moves the probe longitudinally within the probe sheath by telescoping a first sheath element of the plurality within another sheath element of the plurality such that the probe is moved to a position sufficient to measure at least one parameter within the object.

Probe assembly

The invention discloses a bioreactor apparatus () for cultivation of cells comprising: a) a disposable bioreactor vessel () with one or more walls () defining an inner volume (), at least one port () in a wall, wherein the disposable bioreactor vessel is positioned in a rigid support structure (); and b) a heater (), capable of heating an amount of culture medium to a target temperature in the range of 55-95&#xb0; C., while the amount of culture medium is being confined in or conveyed to the inner volume.

Inactivation of viruses

A platform has a filter system with a first set of filter modules and a second set of filter modules that is different from the first set of filter modules. Each set of filter modules includes an inflow channel and an outflow channel. A fluid inlet is connected to the first set of filter modules, a fluid outlet is connected to the second set of filter modules, and a separation interface separates the first and second sets of filter modules. The separation interface has a first interface channel to connect to the module outflow channel of the first set of filter modules, and a second interface channel to connect to the module inflow channel of the second set of filter modules. The filter system receives fluid through the fluid inlet and, after the fluid has passed through each set of filter modules, discharges the fluid through the fluid outlet.

Particle separation and/or purification of a fluid

Disclosed is a jacketed, tiered baffle, bioreactor tank comprising an outer cylindrical-shaped jacket and a cylindrical tank having an inner tank surface defining a chamber configured for supporting a flexible bag disposed within the chamber, and an outer tank surface having tiered baffles configured for routing a heat exchange fluid around the entirety of the outer tank surface, the cylindrical tank disposed axially within the outer cylindrical-shaped jacket. The outer cylindrical-shaped jacket is sealed to the cylindrical tank in a manner sufficient to prevent or minimize loss of the heat exchange fluid.

Temperature controlled support surfaces for single use flexible wall systems

A heat exchange module for use in a chemical, pharmaceutical or biological reactor system includes a generally hollow body having an interior space for receiving a replaceable or single use reactant container, and at least one thermally conductive surface adapted to contact the replaceable reactant container to facilitate heat transfer, a fluid circulation path formed within the body between an outer wall of the body and an inner wall of the body through which a heat exchange fluid can be circulated, and at least one baffle protruding from the inner wall of the body into the interior space.

Temperature controlling surfaces and support structures

Disclosed herein are embodiments of a valve assembly for preventing dead leg spaces in a container or tubing, the embodiments including a three-way valve system for controlling back-pressure in a fluid generating device, such as a single-use high pressure bioreactor. Also disclosed is a pressurized reactor system for bioprocessing, comprising a single-use container including a flexible wall or a semi-rigid wall.

Disposable valve and flexible containers for pressurized bioreactors

A system for condensing moisture in a moist gas stream entering or leaving a bioreactor includes a condenser container, the condenser container comprising: an outer wall surface and an inner wall surface, the inner wall surface defining an interior chamber for holding a fluid; and a first fitment attached to the outer wall surface and forming a first port configured to allow the moist gas stream to flow into the interior chamber; a second fitment attached to the outer wall surface and forming a second port configured to allow a dried gas to flow from the interior chamber and out of the second port; and a cooling device in contact with at least one portion of the outer wall surface and arranged to cool the at least one portion of the outer wall surface.

Disposable bioreactor condenser bag and filter heater

Disclosed is a single-use bioreactor bag design providing substantially equivalent maximum shear rate at the impeller tip and average or bulk shear rate over a range of power per unit working volume and rpm needed for bioculture processing. The uniformity in shear profile of the bag design providing a unique advantage when used as a scale-down or scale-up platform. Also disclosed is a linearly scalable, single-use, bioreactor system for use in carrying out a scalable biomanufacturing process, the system comprising two single-use bioreactor bags of different volumes, wherein the ratio H/D of the height of the working volume H to the diameter D of the tank, or of the bag is equal to about 1.5, and the bulk shear in each of the bags is substantially constant. The disclosed small scale system models larger scale systems from both a shear/scalability/performance basis and also from a validation and regulatory basis. In summary, the 10L bioreactor system disclosed provides for 'linear scalability' by using the same or substantially simulating the same vessel geometry, gas sparging system, impeller shape and type, polymer composition of the flexible bag/components, process control system, and shear rates as larger 50-5000 L systems.

Linearly scalable single use bioreactor system

A probe assembly for inserting a probe into a flexible or semi-rigid vessel or tubing having a distal aseptic connector for coupling to the vessel or tubing, the probe sheath comprising at least a portion that is rigid, the probe sheath extending longitudinally from the aseptic connector and having at least one inner longitudinal lumen configured to receive an elongate sensor or probe body and to permit longitudinal movement of the sensor/probe body within the probe sheath lumen, and an actuator for deploying a probe within the vessel or tubing by advancing the probe body through the aseptic connector to a position where the probe can measure at least one parameter within the vessel or tubing is disclosed herein.

Disclosed herein is a system and method for condensing moisture in a moist gas stream entering a bioreactor or leaving a bioreactor, the system comprising: a condenser container capable of holding a fluid, the condenser container comprising: an outer wall surface and an inner wall surface, the inner wall surface defining an interior chamber for holding the fluid; and a first fitment attached to the outer wall surface of the condenser container, the first fitment forming a first port configured to allow the moist gas stream to flow through the first port and into the interior chamber; a second fitment attached to the outer wall surface of the condenser container, the second fitment forming a second port configured to allow a dried gas to flow from the interior chamber and out of the second port; and a cooling device in contact with at least one portion of the outer wall surface of the condenser container and arranged to cool the at least one portion of the outer wall surface of the condenser container, thereby to condense moisture in the moist gas stream and forming a dry gas stream for entering or leaving the bioreactor.

Growing community of inventors

Marlborough, MA, United States of America

Thomas John Erdenberger