Shoreline Biome, LLC

Thomas Jarvie

Mark Driscoll

Disclosed are methods for lysis of cells that combine three lysis steps&#x2014;(1) heat, (2) detergent and (3) base&#x2014;into a single step and that can be completed in a short period of time, e.g., a few minutes. The methods combine a normally incompatible detergent and base lysis, allow for simplified removal of detergent after lysis, and importantly, limits damage to DNA, such as shearing, that typically results from separate application of conventional lysis methods, yielding improved quality and quantities of genomic DNA (gDNA).

Methods for cell lysis and preparation of high molecular weight DNA from modified cells

Disclosed are methods for screening biological samples for the presence unknown microbes, such as bacteria and archaea or unknown eukaryotes using rRNA gene sequences or other highly conserved genetic regions, across multiple biological samples using a unique sequence tag (barcode) corresponding to the sample. The screening process tracks the unknown microbe or eukaryote in a diluted sample where the DNA has been prepared using whole genome amplification. The whole genome of the unknown microbe or eukaryote is then sequenced and assembled.

High throughput method for identification and sequencing of unknown microbial and eukaryotic genomes from complex mixtures

Disclosed are methods for lysis of cells, such as bacteria present in microbiomes, that combine three lysis steps&#x2014;(1) heat, (2) detergent and (3) base&#x2014;into a single step and that can be completed in a short period of time, e.g., a few minutes. The methods combine a normally incompatible detergent and base lysis, allows for simplified removal of detergent after lysis, and importantly, yields improved quantities of genomic DNA (gDNA) from difficult to lyse bacteria.

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Branford, CT, United States of America

Thomas Jarvie